A guide to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry.

Synaptotagmin I / immunology Humans Flow Cytometry / methods Immunoprecipitation / methods Blotting, Western Fluorescent Antibody Technique / methods Antibodies / immunology
SYT1 Synaptotagmin-1 Uniprot ID P21579 antibody characterization antibody validation immunofluorescence immunoprecipitation western blot

Journal

F1000Research
ISSN: 2046-1402
Titre abrégé: F1000Res
Pays: England
ID NLM: 101594320

Informations de publication

Date de publication:
2024
Historique:
accepted: 11 07 2024
medline: 22 8 2024
pubmed: 22 8 2024
entrez: 22 8 2024
Statut: epublish

Résumé

Synaptotagmin-1 is a synaptic vesicle transmembrane protein that senses calcium influx via its tandem C2-domains, triggering synchronous neurotransmitter release. Disruption to

Identifiants

DOI: 10.12688/f1000research.154034.1 PMID: 39169954 PMC: PMC11336552
pubmed: 39169954
doi: 10.12688/f1000research.154034.1
pmc: PMC11336552
doi:

Substances chimiques

Synaptotagmin I 0
Antibodies 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

817

Informations de copyright

Copyright: © 2024 Biddle MS et al.

Déclaration de conflit d'intérêts

Competing interests: For this project, the laboratory of Peter McPherson developed partnerships with high-quality antibody manufacturers and KO cell line providers. The partners provide antibodies and KO cell lines to the McPherson laboratory at no cost. These partners include: - Abcam- ABCD antibodies- ABclonal- Aviva Systems Biology -Bio Techne -Cell Signalling Technology -Developmental Studies Hybridoma Bank -GeneTex – Horizon Discovery – Proteintech – Synaptic Systems –Thermo Fisher Scientific.

Auteurs

Michael S Biddle (MS)

NIHR BRC-Respiratory, University of Leicester, Leicester, England, UK.
ORCID: 0000-0002-9853-8815

Charles Alende (C)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
ORCID: 0009-0005-4611-6134

Maryam Fotouhi (M)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.

Carolyn Jones (C)

NIHR BRC-Respiratory, University of Leicester, Leicester, England, UK.

Riham Ayoubi (R)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.

Kathleen Southern (K)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
ORCID: 0000-0002-4125-3608

Carl Laflamme (C)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
ORCID: 0000-0001-5906-025X

Harvinder Virk (H)

NIHR BRC-Respiratory, University of Leicester, Leicester, England, UK.
ORCID: 0000-0002-9739-9593

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines du transport vésiculaire › Synaptotagmines › Synaptotagmine I › A guide to selecting high-performing...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A guide to selecting high-performing...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Cytophotométrie › Cytométrie en flux › A guide to selecting high-performing...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Immunoprécipitation › A guide to selecting high-performing...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Immunotransfert › Technique de Western › A guide to selecting high-performing...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Technique d'immunofluorescence › A guide to selecting high-performing...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › A guide to selecting high-performing...