Miniaturized nanoelectrospray interface for coupling capillary electrophoresis with mass spectrometry detection.
capillary electrophoresis
electrospray ionization
mass spectrometry
microfabrication
proteomics
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
23 Aug 2024
23 Aug 2024
Historique:
revised:
17
07
2024
received:
30
04
2024
accepted:
26
07
2024
medline:
23
8
2024
pubmed:
23
8
2024
entrez:
23
8
2024
Statut:
aheadofprint
Résumé
A miniaturized electrospray interface consisting of a microfluidic nanosprayer and nanospray module is reported in the presented short communication. The nanosprayer was fabricated using silicon (Si) technology suitable for cost-efficient high-volume mass production. The nanospray module enabled the positioning of the nanosprayer in front of a mass spectrometry entrance and its coupling with capillary electrophoresis based on the liquid junction principle. A case study of top-down and bottom-up proteomic analyses of intact cytochrome c and its tryptic digest demonstrates the practical applicability of the developed interface.
Identifiants
pubmed: 39177276
doi: 10.1002/elps.202400090
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Technology Agency of the Czech Republic
ID : TO01000232
Organisme : The Czech Science Foundation
ID : GA24-11335J
Informations de copyright
© 2024 The Author(s). Electrophoresis published by Wiley‐VCH GmbH.
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