Phosphoenolpyruvate carboxykinase-2 (PCK2) is a therapeutic target in triple-negative breast cancer.

Artificial intelligence Drug screen Loss of Isozyme Diversity PCK2 TNBC therapy

Journal

Breast cancer research and treatment
ISSN: 1573-7217
Titre abrégé: Breast Cancer Res Treat
Pays: Netherlands
ID NLM: 8111104

Informations de publication

Date de publication:
23 Aug 2024
Historique:
received: 21 04 2024
accepted: 07 08 2024
medline: 23 8 2024
pubmed: 23 8 2024
entrez: 23 8 2024
Statut: aheadofprint

Résumé

Metabolic rewiring in malignant transformation is often accompanied by altered expression of metabolic isozymes. Phosphoenolpyruvate carboxykinase-2 (PCK2) catalyzes the rate-limiting step of gluconeogenesis and is the dominant isoform in many cancers including triple-negative breast cancer (TNBC). Our goal was to identify small molecule inhibitors of PCK2 enzyme activity. We assessed the impact of PCK2 down regulation with shRNA on TNBC cell growth in vitro and used AtomNet® deep convolutional neural network software to identify potential small molecule inhibitors of PCK2-based structure. We iteratively tested candidate compounds in an in vitro PCK-2 enzyme assay. The impact of the top hit on metabolic flux and cell viability was also assessed. PCK2 downregulation decreased growth of BT-549 and MDA-MB-231 cells and reduced metabolic flux through pyruvate carboxylase. The first AtomNet® in silico structural screen of 7 million compounds yielded 86 structures that were tested in PCK2 enzyme assay in vitro. The top hit (IC 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate is a high affinity PCK2 enzyme inhibitor that also has significant growth inhibitory activity in breast cell lines in vitro and represents a potential therapeutic lead compound.

Identifiants

pubmed: 39177932
doi: 10.1007/s10549-024-07462-z
pii: 10.1007/s10549-024-07462-z
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Breast Cancer Research Foundation investigator award
ID : BCRF-22-133
Organisme : Susan Komen Leadership Grant
ID : SAC220225

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Vignesh Gunasekharan (V)

Yale Cancer Center, Yale School of Medicine, 300 George Street, Suite 120, Rm 133, New Haven, CT, 06511, USA.

Hao-Kuen Lin (HK)

Yale Cancer Center, Yale School of Medicine, 300 George Street, Suite 120, Rm 133, New Haven, CT, 06511, USA.

Michal Marczyk (M)

Yale Cancer Center, Yale School of Medicine, 300 George Street, Suite 120, Rm 133, New Haven, CT, 06511, USA.
Department of Data Science and Engineering, Silesian University of Technology, Gliwice, Poland.

Alejandro Rios-Hoyo (A)

Yale Cancer Center, Yale School of Medicine, 300 George Street, Suite 120, Rm 133, New Haven, CT, 06511, USA.

Gerson Espinoza Campos (GE)

Yale Cancer Center, Yale School of Medicine, 300 George Street, Suite 120, Rm 133, New Haven, CT, 06511, USA.

Naing Lin Shan (NL)

Yale Cancer Center, Yale School of Medicine, 300 George Street, Suite 120, Rm 133, New Haven, CT, 06511, USA.

Mostafa Ahmed (M)

Atomwise, San Francisco, CA, USA.

Sheila Umlauf (S)

Yale Center for Molecular Discovery, Yale University, West Haven, CT, USA.

Peter Gareiss (P)

Yale Center for Molecular Discovery, Yale University, West Haven, CT, USA.

Raaisa Raaisa (R)

Yale Cancer Center, Yale School of Medicine, 300 George Street, Suite 120, Rm 133, New Haven, CT, 06511, USA.

Richard Williams (R)

Yale Cancer Center, Yale School of Medicine, 300 George Street, Suite 120, Rm 133, New Haven, CT, 06511, USA.

Rebecca Cardone (R)

Yale Cancer Center, Yale School of Medicine, 300 George Street, Suite 120, Rm 133, New Haven, CT, 06511, USA.

Stephan Siebel (S)

Yale Cancer Center, Yale School of Medicine, 300 George Street, Suite 120, Rm 133, New Haven, CT, 06511, USA.

Richard Kibbey (R)

Yale Cancer Center, Yale School of Medicine, 300 George Street, Suite 120, Rm 133, New Haven, CT, 06511, USA.

Yulia V Surovtseva (YV)

Yale Center for Molecular Discovery, Yale University, West Haven, CT, USA.

Lajos Pusztai (L)

Yale Cancer Center, Yale School of Medicine, 300 George Street, Suite 120, Rm 133, New Haven, CT, 06511, USA. lajos.pusztai@yale.edu.

Classifications MeSH