Differential CheR Affinity for Chemoreceptor C-Terminal Pentapeptides Modulates Chemotactic Responses.
CheR
chemoreceptor
chemotaxis
molecular recognition
pentapeptide
signal transduction
Journal
Molecular microbiology
ISSN: 1365-2958
Titre abrégé: Mol Microbiol
Pays: England
ID NLM: 8712028
Informations de publication
Date de publication:
23 Aug 2024
23 Aug 2024
Historique:
revised:
25
07
2024
received:
09
05
2024
accepted:
27
07
2024
medline:
24
8
2024
pubmed:
24
8
2024
entrez:
24
8
2024
Statut:
aheadofprint
Résumé
Many chemoreceptors contain a C-terminal pentapeptide at the end of a linker. In Escherichia coli, this pentapeptide forms a high-affinity binding site for CheR and phosphorylated CheB, and its removal interferes with chemoreceptor adaptation. Analysis of chemoreceptors revealed significant variation in their pentapeptide sequences, and bacteria often possess multiple chemoreceptors with differing pentapeptides. To assess whether this sequence variation alters CheR affinity and chemotaxis, we used Pectobacterium atrosepticum SCRI1043 as a model. SCRI1043 has 36 chemoreceptors, with 19 of them containing a C-terminal pentapeptide. We show that the affinity of CheR for the different pentapeptides varies up to 11-fold (K
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Consejo Superior de Investigaciones Científicas
ID : 2023AEP002
Organisme : Junta de Andalucía
ID : P18-FR-1621
Organisme : Ministerio de Ciencia e Innovación
ID : PID2019-103972GA-I00
Organisme : Ministerio de Ciencia e Innovación
ID : PID2020-112612GB-I00
Informations de copyright
© 2024 John Wiley & Sons Ltd.
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