The significance of electrical signals in maturing spermatozoa for phosphoinositide regulation through voltage-sensing phosphatase.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
24 Aug 2024
24 Aug 2024
Historique:
received:
29
04
2024
accepted:
14
08
2024
medline:
26
8
2024
pubmed:
26
8
2024
entrez:
24
8
2024
Statut:
epublish
Résumé
Voltage-sensing phosphatase (VSP) exhibits voltage-dependent phosphatase activity toward phosphoinositides. VSP generates a specialized phosphoinositide environment in mammalian sperm flagellum. However, the voltage-sensing mechanism of VSP in spermatozoa is not yet characterized. Here, we found that VSP is activated during sperm maturation, indicating that electric signals in immature spermatozoa are essential. Using a heterologous expression system, we show the voltage-sensing property of mouse VSP (mVSP). The voltage-sensing threshold of mVSP is approximately -30 mV, which is sensitive enough to activate mVSP in immature spermatozoa. We also report several knock-in mice in which we manipulate the voltage-sensitivity or electrochemical coupling of mVSP. Notably, the V312R mutant, with a minor voltage-sensitivity change, exhibits abnormal sperm motility after, but not before, capacitation. Additionally, the V312R mutant shows a significant change in the acyl-chain profile of phosphoinositide. Our findings suggest that electrical signals during sperm maturation are crucial for establishing the optimal phosphoinositide environment in spermatozoa.
Identifiants
pubmed: 39181879
doi: 10.1038/s41467-024-51755-2
pii: 10.1038/s41467-024-51755-2
doi:
Substances chimiques
Phosphatidylinositols
0
Phosphoric Monoester Hydrolases
EC 3.1.3.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7289Subventions
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 20KK0376
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 17K15558
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 20K07274
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 15H05901
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 21229003
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 25253016
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 19H03401
Organisme : Ichiro Kanehara Foundation for the Promotion of Medical Sciences and Medical Care (Ichiro Kanehara Foundation)
ID : n.a
Organisme : Sumitomo Foundation
ID : n.a
Organisme : Ono Medical Research Foundation
ID : n.a
Organisme : Uehara Memorial Foundation
ID : n.a
Organisme : Senri Life Science Foundation
ID : n.a
Organisme : Takeda Science Foundation
ID : n.a
Organisme : Mochida Memorial Foundation for Medical and Pharmaceutical Research
ID : n.a
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : 24gm1710007
Organisme : Tokyo Medical and Dental University (TMDU)
ID : JPMXP1323015483
Informations de copyright
© 2024. The Author(s).
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