Chemical composition of essential and fixed oils of Tagetes erecta fruits (Iran) and their implications in inhibition of cancer signaling.
Humans
Oils, Volatile
/ pharmacology
Signal Transduction
/ drug effects
Tagetes
/ chemistry
Fruit
/ chemistry
Cell Line, Tumor
Iran
Proto-Oncogene Proteins c-akt
/ metabolism
Plant Oils
/ pharmacology
TOR Serine-Threonine Kinases
/ metabolism
Antineoplastic Agents, Phytogenic
/ pharmacology
Phosphatidylinositol 3-Kinases
/ metabolism
Gas Chromatography-Mass Spectrometry
Cell Proliferation
/ drug effects
Tagetes erecta fruit
Cytotoxic
Essential oil
Fatty acid composition
PI3K/AKT/mTOR
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
24 08 2024
24 08 2024
Historique:
received:
29
04
2024
accepted:
19
08
2024
medline:
26
8
2024
pubmed:
26
8
2024
entrez:
24
8
2024
Statut:
epublish
Résumé
The current research was conducted to explore, for the first time, Tagetes erecta L. (family Asteraceae) fruits from northwest Iran in terms of the chemical composition of essential and fixed oils, their cytotoxic activities, and the inhibitory effect of essential oil on the PI3K/AKT/mTOR signaling pathway. The volatile oil was obtained through hydrodistillation (Clevenger apparatus). According to gas chromatography-mass spectrometry analysis, the essential oil was rich in cyclic monoterpenoids, 2-isopropyl-5-methyl-3-cyclohexen-1-one (19.99%), D-limonene (12.75%), terpinolene (11.64%) and also the saturated fatty acid palmitic acid (19.09%). Furthermore, the seeds of T. erecta were extracted using hexane by the maceration method. The analysis of fatty acid profile of the fixed oil by gas chromatography-flame ionization detector (GC-FID) demonstrated that the most predominant fatty acids in fixed oil were linoleic acid (59.53%), palmitic acid (13.70%), stearic acid (10.20%), and oleic acid (9.20%). The cytotoxic activity of essential oil, crude oil, and fraction A (obtained from fixed oil) were evaluated by using the MTT assay on MCF7 (human breast cancer cell line), PC3 (human prostate cancer cell line), and U87MG (human glioblastoma cell line). Finally, the effect of essential oil on inhibiting the PI3K/Akt/mTOR signaling pathway was evaluated using real-time PCR. The essential oil exhibited vigorous cytotoxic activity on the U87MG cell line, with an IC
Identifiants
pubmed: 39181940
doi: 10.1038/s41598-024-70582-5
pii: 10.1038/s41598-024-70582-5
doi:
Substances chimiques
Oils, Volatile
0
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Plant Oils
0
TOR Serine-Threonine Kinases
EC 2.7.11.1
Antineoplastic Agents, Phytogenic
0
Phosphatidylinositol 3-Kinases
EC 2.7.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
19667Informations de copyright
© 2024. The Author(s).
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