Efficient biosynthesis of D/L-alanine in the recombinant

D-glucose D/L-alanine SDS-PAGE biobrick method gene expression optimization whole cell catalysis

Journal

Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513

Informations de publication

Date de publication:
2024
Historique:
received: 21 04 2024
accepted: 26 07 2024
medline: 27 8 2024
pubmed: 27 8 2024
entrez: 27 8 2024
Statut: epublish

Résumé

Alanine is the most abundant chiral amino acid that exists into the D-alanine or L-alanine forms with diverse applications in the biomedical, pharmaceutical, plastics, and food industries. D/L-alanine production can be carried out through chemical, microbial fermentation, and biocatalytic methods and not much effective due to complicated processes or purification issues and is still challenging to achieve a higher yield. In the present study, biobrick method was utilized for efficient production of D/L-alanine in the recombinant

Identifiants

pubmed: 39188373
doi: 10.3389/fbioe.2024.1421167
pii: 1421167
pmc: PMC11345225
doi:

Types de publication

Journal Article

Langues

eng

Pagination

1421167

Informations de copyright

Copyright © 2024 Naeem, Hao, Chu, Zhang, Huang, Wang, He, Zhao and Ju.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Auteurs

Muhammad Naeem (M)

College of Life Science, Hebei Normal University, Shijiazhuang, China.

Shimiao Hao (S)

College of Life Science, Hebei Normal University, Shijiazhuang, China.
Hebei Collaborative Innovation Center for Eco-Environment, Shijiazhuang, China.

Mengqiu Chu (M)

College of Life Science, Hebei Normal University, Shijiazhuang, China.

Xuan Zhang (X)

College of Life Science, Hebei Normal University, Shijiazhuang, China.

Xinyan Huang (X)

College of Life Science, Hebei Normal University, Shijiazhuang, China.

Jiaying Wang (J)

College of Life Science, Hebei Normal University, Shijiazhuang, China.

Guangzheng He (G)

College of Life Science, Hebei Normal University, Shijiazhuang, China.

Baohua Zhao (B)

College of Life Science, Hebei Normal University, Shijiazhuang, China.

Jiansong Ju (J)

College of Life Science, Hebei Normal University, Shijiazhuang, China.
Hebei Collaborative Innovation Center for Eco-Environment, Shijiazhuang, China.

Classifications MeSH