Evaluation of Th1/Th2/Th17 Balance in Pulmonary Cystic Echinococcosis Patients.
PBMC
Pulmonary Cystic Echinococcosis
Th1
Th17
Th2
Journal
Acta parasitologica
ISSN: 1896-1851
Titre abrégé: Acta Parasitol
Pays: Switzerland
ID NLM: 9301947
Informations de publication
Date de publication:
27 Aug 2024
27 Aug 2024
Historique:
received:
26
06
2024
accepted:
13
08
2024
medline:
27
8
2024
pubmed:
27
8
2024
entrez:
27
8
2024
Statut:
aheadofprint
Résumé
Cystic echinococcosis (CE) is a neglected tropical disease prevalent worldwide, particularly in rural areas. Previous studies evaluated immune responses in patients with hepatic CE, however none had assessed Th1, Th2 and Th17 levels simultaneously in pulmonary CE patients. This study aimed to fill this gap in literature by using flow cytometry analysis. Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples collected from healthy control (HC) volunteers and patients with active pulmonary CE cysts. The PBMCs were analysed to evaluate Th1, Th2, and Th17 cell levels within the CD3 + CD4 + T-cell population, using antibodies against interferon (IFN)-γ, interleukin (IL)-4, and IL-17, respectively. Our analysis revealed elevated Th2 levels in CE patients, while Th1 and Th17 cell counts showed no significant difference between HC volunteers and patients with pulmonary CE. The results indicate an imbalanced Th1/Th2/Th17 cell regulation in the pathogenesis of pulmonary CE. Future studies are recommended to compare immune responses between pulmonary and hepatic CE to confirm these findings and evaluate any potential difference in the immunopathology associated with the two clinical forms of CE.
Identifiants
pubmed: 39190279
doi: 10.1007/s11686-024-00907-x
pii: 10.1007/s11686-024-00907-x
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Hitit University Scientific Research Projects Coordination Unit
ID : TIP19001.20.003
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
Références
WHO– World Health Organization (2018) Echinococcosis. In: Facts Sheets. http://www.who.int/news-room/fact-sheets/detail/echinococcosis
Sarkar M, Pathania R, Jhobta A et al (2016) Cystic pulmonary hydatidosis. Lung India 33. https://doi.org/10.4103/0970-2113.177449
CDC (2016) CDC - DPDx - Echinococcosis. Glob. Heal.– div. Parasit. Dis. Malar
Grubor NM, Jovanova-Nesic KD, Shoenfeld Y (2017) Liver cystic echinococcosis and human host immune and autoimmune follow-up: a review. World J Hepatol 9:1176–1189
doi: 10.4254/wjh.v9.i30.1176
pubmed: 29109850
pmcid: 5666304
Mourglia-Ettlin G, Marqués JM, Chabalgoity JA, Dematteis S (2011) Early peritoneal immune response during Echinococcus granulosus establishment displays a biphasic behavior. PLoS Negl Trop Dis 5. https://doi.org/10.1371/journal.pntd.0001293
Rostami-Rad S, Jafari R, Yousofi Darani H (2018) Th1/Th2-type cytokine profile in C57 black mice inoculated with live Echinococcus granulosus protoscolices. J Infect Public Health 11. https://doi.org/10.1016/j.jiph.2018.06.007
Mezioug D, Touil-Boukoffa C (2012) Interleukin-17A correlates with interleukin-6 production in human cystic echinococcosis: a possible involvement of IL-17A in immunoprotection against echinococcus granulosus infection. Eur Cytokine Netw 23. https://doi.org/10.1684/ecn.2012.0314
Yasen A, Li W, Aini A et al (2021) Th1/Th2/Th17 cytokine profile in hepatic cystic echinococcosis patients with different cyst stages. Parasite Immunol 43. https://doi.org/10.1111/pim.12839
Amni F, Hajizadeh M, Elmi T et al (2021) Different manifestation of Echinococcus Granulosus immunogenic antigens in the liver and lungs of intermediate host. Comp Immunol Microbiol Infect Dis 74:101573. https://doi.org/10.1016/j.cimid.2020.101573
doi: 10.1016/j.cimid.2020.101573
pubmed: 33189997
Zhang W, Wen H, Li J et al (2012) Immunology and immunodiagnosis of cystic echinococcosis: An update. Clin. Dev. Immunol. 2012
Macpherson CNL, Vuitton DA, Gharbi HA et al (2003) International classification of ultrasound images in cystic echinococcosis for application in clinical and field epidemiological settings. In: Acta Tropica
Gazi U, Yapar D, Karasartova D et al (2018) The role of Treg population in pathogenesis of Crimean Congo hemorrhagic fever. Virus Res 250:1–6. https://doi.org/10.1016/j.virusres.2018.04.003
doi: 10.1016/j.virusres.2018.04.003
pubmed: 29625147
Karasartova D, Gazi U, Tosun O et al (2017) Anti-pneumococcal vaccine-induced cellular immune responses in post-traumatic splenectomized individuals. J Clin Immunol 37:388–396. https://doi.org/10.1007/s10875-017-0397-3
doi: 10.1007/s10875-017-0397-3
pubmed: 28488145
Naik MI, Tenguria RK, Haq E (2016) Detection of serum cytokines before and after pharmacological and surgical treatment in patients with cystic echinococcosis. J Helminthol 90. https://doi.org/10.1017/S0022149X15000085
Biranvand E, Rafiei A, Beiromvand M et al (2020) Cytokine profiles in peripheral blood mononuclear cells from patients with cystic echinococcosis. Comp Immunol Microbiol Infect Dis 70. https://doi.org/10.1016/j.cimid.2020.101469
Riganò R, Buttari B, De Falco E et al (2004) Echinococcus granulosus-specific T-cell lines derived from patients at various clinical stages of cystic echinococcosis. Parasite Immunol 26:45–52. https://doi.org/10.1111/j.0141-9838.2004.00682.x
doi: 10.1111/j.0141-9838.2004.00682.x
pubmed: 15198645
Amri M, Mezioug D, Touil-Boukoffa C (2009) Involvement of IL-10 and IL-4 in evasion strategies of Echinococcus granulosus to host immune response. Eur Cytokine Netw 20:63–68. https://doi.org/10.1684/ecn.2009.0154
doi: 10.1684/ecn.2009.0154
pubmed: 19541591
Piccoli L, Meroni V, Genco F et al (2012) Serum cytokine profile by ELISA in patients with echinococcal cysts of the liver: a stage-specific approach to assess their biological activity. Clin Dev Immunol 2012. https://doi.org/10.1155/2012/483935
Tamarozzi F, Meroni V, Genco F et al (2010) Ex vivo assessment of serum cytokines in patients with cystic echinococcosis of the liver. Parasite Immunol 32. https://doi.org/10.1111/j.1365-3024.2010.01236.x
Petrone L, Vanini V, Petruccioli E et al (2015) Polyfunctional specific response to Echinococcus Granulosus associates to the biological activity of the cysts. PLoS Negl Trop Dis 9. https://doi.org/10.1371/journal.pntd.0004209
Fratini F, Tamarozzi F, Macchia G et al (2020) Proteomic analysis of plasma exosomes from cystic echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers. PLoS Negl Trop Dis 14. https://doi.org/10.1371/journal.pntd.0008586
Petrone L, Vanini V, Petruccioli E et al (2015) IL-4 specific-response in whole blood associates with human cystic echinococcosis and cyst activity. J Infect 70. https://doi.org/10.1016/j.jinf.2014.10.009
Li ZD, Mo XJ, Yan S et al (2020) Multiplex cytokine and antibody profile in cystic echinococcosis patients during a three-year follow-up in reference to the cyst stages. Parasites Vectors 13. https://doi.org/10.1186/s13071-020-4003-9
Tuxun T, Wang JH, Lin RY et al (2012) Th17/Treg imbalance in patients with liver cystic echinococcosis. Parasite Immunol 34. https://doi.org/10.1111/j.1365-3024.2012.01383.x
Sadjjadi SM, Mikaeili F, Karamian M et al (2013) Evidence that the Echinococcus granulosus G6 genotype has an affinity for the brain in humans. Int J Parasitol 43:875–877. https://doi.org/10.1016/j.ijpara.2013.06.008
doi: 10.1016/j.ijpara.2013.06.008
pubmed: 23891711