Retrospective ANalysis of multi-drug resistant Gram-nEgative bacteRia on veno-venous extracorporeal membrane oxygenation. The multicenter RANGER STUDY.


Journal

Critical care (London, England)
ISSN: 1466-609X
Titre abrégé: Crit Care
Pays: England
ID NLM: 9801902

Informations de publication

Date de publication:
27 Aug 2024
Historique:
received: 26 06 2024
accepted: 15 08 2024
medline: 28 8 2024
pubmed: 28 8 2024
entrez: 27 8 2024
Statut: epublish

Résumé

Veno-venous extracorporeal membrane oxygenation (V-V ECMO) is a rapidly expanding life-support technique worldwide. The most common indications are severe hypoxemia and/or hypercapnia, unresponsive to conventional treatments, primarily in cases of acute respiratory distress syndrome. Concerning potential contraindications, there is no mention of microbiological history, especially related to multi-drug resistant (MDR) bacteria isolated before V-V ECMO placement. Our study aims to investigate: (i) the prevalence and incidence of MDR Gram-negative (GN) bacteria in a cohort of V-V ECMOs; (ii) the risk of 1-year mortality, especially in the case of predetected MDR GN bacteria; and (iii) the impact of annual hospital V-V ECMO volume on the probability of acquiring MDR GN bacteria. All consecutive adults admitted to the Intensive Care Units of 5 Italian university-affiliated hospitals and requiring V-V ECMO were screened. Exclusion criteria were age < 18 years, pregnancy, veno-arterial or mixed ECMO-configuration, incomplete records, survival < 24 h after V-V ECMO. A standard protocol of microbiological surveillance was applied and MDR profiles were identified using in vitro susceptibility tests. Cox-proportional hazards models were applied for investigating mortality. Two hundred and seventy-nine V-V ECMO patients (72% male) were enrolled. The overall MDR GN bacteria percentage was 50%: 21% (n.59) detected before and 29% (n.80) after V-V ECMO placement. The overall 1-year mortality was 42%, with a higher risk observed in predetected patients (aHR 2.14 [1.33-3.47], p value 0.002), while not in 'V-V ECMO-acquired MDR GN bacteria' group (aHR 1.51 [0.94-2.42], p value 0.090), as compared to 'non-MDR GN bacteria' group (reference). Same findings were found considering only infections. A larger annual hospital V-V ECMO volume was associated with a lower probability of acquiring MDR GN bacteria during V-V ECMO course (aOR 0.91 [0.86-0.97], p value 0.002). 21% of MDR GN bacteria were detected before; while 29% after V-V ECMO connection. A history of MDR GN bacteria, isolated before V-V ECMO, was an independent risk factor for mortality. The annual hospital V-V ECMO volume affected the probability of acquiring MDR GN bacteria. Trial Registration ClinicalTrial.gov Registration Number NCTNCT06199141, date 12.26.2023.

Sections du résumé

BACKGROUND BACKGROUND
Veno-venous extracorporeal membrane oxygenation (V-V ECMO) is a rapidly expanding life-support technique worldwide. The most common indications are severe hypoxemia and/or hypercapnia, unresponsive to conventional treatments, primarily in cases of acute respiratory distress syndrome. Concerning potential contraindications, there is no mention of microbiological history, especially related to multi-drug resistant (MDR) bacteria isolated before V-V ECMO placement. Our study aims to investigate: (i) the prevalence and incidence of MDR Gram-negative (GN) bacteria in a cohort of V-V ECMOs; (ii) the risk of 1-year mortality, especially in the case of predetected MDR GN bacteria; and (iii) the impact of annual hospital V-V ECMO volume on the probability of acquiring MDR GN bacteria.
METHODS METHODS
All consecutive adults admitted to the Intensive Care Units of 5 Italian university-affiliated hospitals and requiring V-V ECMO were screened. Exclusion criteria were age < 18 years, pregnancy, veno-arterial or mixed ECMO-configuration, incomplete records, survival < 24 h after V-V ECMO. A standard protocol of microbiological surveillance was applied and MDR profiles were identified using in vitro susceptibility tests. Cox-proportional hazards models were applied for investigating mortality.
RESULTS RESULTS
Two hundred and seventy-nine V-V ECMO patients (72% male) were enrolled. The overall MDR GN bacteria percentage was 50%: 21% (n.59) detected before and 29% (n.80) after V-V ECMO placement. The overall 1-year mortality was 42%, with a higher risk observed in predetected patients (aHR 2.14 [1.33-3.47], p value 0.002), while not in 'V-V ECMO-acquired MDR GN bacteria' group (aHR 1.51 [0.94-2.42], p value 0.090), as compared to 'non-MDR GN bacteria' group (reference). Same findings were found considering only infections. A larger annual hospital V-V ECMO volume was associated with a lower probability of acquiring MDR GN bacteria during V-V ECMO course (aOR 0.91 [0.86-0.97], p value 0.002).
CONCLUSIONS CONCLUSIONS
21% of MDR GN bacteria were detected before; while 29% after V-V ECMO connection. A history of MDR GN bacteria, isolated before V-V ECMO, was an independent risk factor for mortality. The annual hospital V-V ECMO volume affected the probability of acquiring MDR GN bacteria. Trial Registration ClinicalTrial.gov Registration Number NCTNCT06199141, date 12.26.2023.

Identifiants

pubmed: 39192287
doi: 10.1186/s13054-024-05068-x
pii: 10.1186/s13054-024-05068-x
doi:

Banques de données

ClinicalTrials.gov
['NCT06199141']

Types de publication

Journal Article Multicenter Study

Langues

eng

Sous-ensembles de citation

IM

Pagination

279

Informations de copyright

© 2024. The Author(s).

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Auteurs

Annalisa Boscolo (A)

Department of Medicine (DIMED), University of Padua, 13 Gallucci Street, 35121, Padua, Italy.
Institute of Anesthesia and Critical Care, Padua University Hospital, Padua, Italy.
Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padua, Padua, Italy.

Andrea Bruni (A)

Department of Medical and Surgical Sciences, Magna Graecia University, Catanzaro, Italy.

Marco Giani (M)

School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy.
Department of Emergency and Critical Care, IRCSS San Gerardo Dei Tintori, Monza, Italy.

Eugenio Garofalo (E)

Department of Medical and Surgical Sciences, Magna Graecia University, Catanzaro, Italy.

Nicolò Sella (N)

Institute of Anesthesia and Critical Care, Padua University Hospital, Padua, Italy.

Tommaso Pettenuzzo (T)

Institute of Anesthesia and Critical Care, Padua University Hospital, Padua, Italy.

Michela Bombino (M)

Department of Emergency and Critical Care, IRCSS San Gerardo Dei Tintori, Monza, Italy.

Matteo Palcani (M)

School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy.

Emanuele Rezoagli (E)

School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy.
Department of Emergency and Critical Care, IRCSS San Gerardo Dei Tintori, Monza, Italy.

Matteo Pozzi (M)

School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy.
Department of Emergency and Critical Care, IRCSS San Gerardo Dei Tintori, Monza, Italy.

Elena Falcioni (E)

Department of Surgery, Dentistry, Paediatrics and Gynaecology, University of Verona, Verona, Italy.
Cardiothoracic and Vascular Intensive Care Unit, Verona University Hospital, Verona, Italy.

Elisa Pistollato (E)

Department of Medicine (DIMED), University of Padua, 13 Gallucci Street, 35121, Padua, Italy.

Eugenio Biamonte (E)

Department of Medical and Surgical Sciences, Magna Graecia University, Catanzaro, Italy.

Francesco Murgolo (F)

Department of Precision and Regenerative Medicine and Ionian Area, School of Medicine, University of Bari "Aldo Moro", Bari, Italy.

Graziella D'Arrigo (G)

CNR-IFC, Institute of Clinical Physiology of Reggio Calabria, Reggio Calabria, Italy.

Mercedes Gori (M)

CNR-IFC, Institute of Clinical Physiology of Rome, Rome, Italy.

Giovanni Luigi Tripepi (GL)

CNR-IFC, Institute of Clinical Physiology of Reggio Calabria, Reggio Calabria, Italy.

Leonardo Gottin (L)

Department of Surgery, Dentistry, Paediatrics and Gynaecology, University of Verona, Verona, Italy.
Cardiothoracic and Vascular Intensive Care Unit, Verona University Hospital, Verona, Italy.

Federico Longhini (F)

Department of Medical and Surgical Sciences, Magna Graecia University, Catanzaro, Italy.

Salvatore Grasso (S)

Department of Precision and Regenerative Medicine and Ionian Area, School of Medicine, University of Bari "Aldo Moro", Bari, Italy.

Paolo Navalesi (P)

Department of Medicine (DIMED), University of Padua, 13 Gallucci Street, 35121, Padua, Italy. paolo.navalesi@unipd.it.
Institute of Anesthesia and Critical Care, Padua University Hospital, Padua, Italy. paolo.navalesi@unipd.it.

Giuseppe Foti (G)

School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy.
Department of Emergency and Critical Care, IRCSS San Gerardo Dei Tintori, Monza, Italy.

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