Evaluating stability and bioactivity of Rehmannia-derived nanovesicles during storage.
Bioactivity
Plant derived nanovesicles
Rehmannia
Stability
Storage
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 08 2024
28 08 2024
Historique:
received:
28
03
2024
accepted:
14
08
2024
medline:
31
8
2024
pubmed:
31
8
2024
entrez:
28
8
2024
Statut:
epublish
Résumé
Plant-derived nanovesicles (PDNVs) have garnered growing attention in the biomedical field owing to their abundance in plant-derived ribonucleic acids (RNA), proteins, lipids and metabolites. The question about the preservation of PDNVs is a crucial and unavoidable concern in both experiments' settings and their potential clinical application. The objective of this research was to examine the impact of varying storage temperatures on the stability and bioactivity of Rehmannia-derived nanovesicles (RDNVs). The results showed that RDNVs aggregated after 2 weeks of storage period at 4 °C, and the particle size of some RDNVs gradually increased with time, along with the increase of solution potential. After 2 months of storage, all RDNVs exhibited varying levels of aggregation irrespective of storage temperature. The bioactivities of nanovesicles under different temperature storage conditions revealed a gradual decline in cell proliferation inhibition bioactivity over time, significantly lower than that of freshly prepared RDNVs. In contrast, the preservation of anti-migratory activity in RDNVs was found to be more effective when subjected to rapid freezing in liquid nitrogen followed by storage at - 80 °C, as opposed to direct storage at - 80 °C. These findings suggest that temperature alone may not be sufficient in safeguarding the activity and stability of RDNVs, highlighting the necessity for the development of novel protective agents for PDNVs.
Identifiants
pubmed: 39198513
doi: 10.1038/s41598-024-70334-5
pii: 10.1038/s41598-024-70334-5
doi:
Substances chimiques
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
19966Subventions
Organisme : the Startup Fund for scientific research, Fujian Medical University
ID : 2023QH2014
Organisme : Special Project of Central Government for Local Science and Technology Development of Fujian Province
ID : 2023L3011
Organisme : National Natural Science Foundation of China
ID : 82173180
Organisme : National Natural Science Foundation of China
ID : 82272868
Informations de copyright
© 2024. The Author(s).
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