Average Nucleotide Identity and Digital DNA-DNA Hybridization Analysis Following PromethION Nanopore-Based Whole Genome Sequencing Allows for Accurate Prokaryotic Typing.

Escherichia coli antibiotic resistance average nucleotide identity clinical bacteriology digital DNA-DNA hybridization minimum inhibitory concentration multi-locus sequence typing nanopore sequencing species delineation whole genome sequencing

Journal

Diagnostics (Basel, Switzerland)
ISSN: 2075-4418
Titre abrégé: Diagnostics (Basel)
Pays: Switzerland
ID NLM: 101658402

Informations de publication

Date de publication:
17 Aug 2024
Historique:
received: 31 05 2024
revised: 31 07 2024
accepted: 13 08 2024
medline: 31 8 2024
pubmed: 31 8 2024
entrez: 29 8 2024
Statut: epublish

Résumé

Whole-genome sequencing (WGS) is revolutionizing clinical bacteriology. However, bacterial typing remains investigated by reference techniques with inherent limitations. This stresses the need for alternative methods providing robust and accurate sequence type (ST) classification. This study optimized and evaluated a GridION nanopore sequencing protocol, adapted for the PromethION platform. Forty-eight

Identifiants

pubmed: 39202288
pii: diagnostics14161800
doi: 10.3390/diagnostics14161800
pmc: PMC11353866
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Research Foundation - Flanders
ID : 1S97421N
Organisme : Special Research Fund of Ghent University
ID : BOF.COV.2020.0009.01

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Auteurs

Nick Versmessen (N)

Laboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.
Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.

Marieke Mispelaere (M)

Department of Bio-Medical Sciences, HOWEST University of Applied Sciences, 8000 Bruges, Belgium.

Marjolein Vandekerckhove (M)

Department of Bio-Medical Sciences, HOWEST University of Applied Sciences, 8000 Bruges, Belgium.

Cedric Hermans (C)

Department of Bio-Medical Sciences, HOWEST University of Applied Sciences, 8000 Bruges, Belgium.

Jerina Boelens (J)

Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.
Department of Laboratory Medicine, Ghent University Hospital, 9000 Ghent, Belgium.

Katleen Vranckx (K)

BioMérieux, 1030 Brussels, Belgium.

Filip Van Nieuwerburgh (F)

NXTGNT, Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Ghent University, 9000 Ghent, Belgium.

Mario Vaneechoutte (M)

Laboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.

Paco Hulpiau (P)

Department of Bio-Medical Sciences, HOWEST University of Applied Sciences, 8000 Bruges, Belgium.

Piet Cools (P)

Laboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.
Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.

Classifications MeSH