Molecular Investigation of Small Ruminant Abortions Using a 10-Plex HRM-qPCR Technique: A Novel Approach in Routine Diagnostics.
Anaplasma phagocytophilum
Brucella spp.
Campylobacter fetus
Chlamydophila spp.
Coxiella burnetii
Greece
HRM
Neospora caninum
Salmonella spp.
Toxoplasma gondii
abortogenic pathogens
qPCR
small ruminants
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
14 Aug 2024
14 Aug 2024
Historique:
received:
19
07
2024
revised:
04
08
2024
accepted:
13
08
2024
medline:
31
8
2024
pubmed:
31
8
2024
entrez:
29
8
2024
Statut:
epublish
Résumé
The objective of this study was to apply and preliminarily evaluate a High-Resolution Melting (HRM) analysis technique coupled with qPCR, that allows the simultaneous detection of 10 different ruminant abortogenic pathogens, for investigating abortions in sheep and goats throughout Greece. A total of 264 ovine and caprine vaginal swabs were obtained the week following the abortion from aborted females and analyzed using a commercially available kit (ID Gene™ Ruminant Abortion Multiplex HRM, Innovative Diagnostics). Results indicated a high prevalence of
Identifiants
pubmed: 39203517
pii: microorganisms12081675
doi: 10.3390/microorganisms12081675
pmc: PMC11356958
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : CEVA Hellas
ID : 7652 (University of Thessaly)
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