Mitochondrial double-stranded RNA homeostasis depends on cell-cycle progression.
Journal
Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869
Informations de publication
Date de publication:
Nov 2024
Nov 2024
Historique:
received:
10
04
2024
revised:
19
08
2024
accepted:
21
08
2024
medline:
31
8
2024
pubmed:
31
8
2024
entrez:
29
8
2024
Statut:
epublish
Résumé
Mitochondrial gene expression is a compartmentalised process essential for metabolic function. The replication and transcription of mitochondrial DNA (mtDNA) take place at nucleoids, whereas the subsequent processing and maturation of mitochondrial RNA (mtRNA) and mitoribosome assembly are localised to mitochondrial RNA granules. The bidirectional transcription of circular mtDNA can lead to the hybridisation of polycistronic transcripts and the formation of immunogenic mitochondrial double-stranded RNA (mt-dsRNA). However, the mechanisms that regulate mt-dsRNA localisation and homeostasis are largely unknown. With super-resolution microscopy, we show that mt-dsRNA overlaps with the RNA core and associated proteins of mitochondrial RNA granules but not nucleoids. Mt-dsRNA foci accumulate upon the stimulation of cell proliferation and their abundance depends on mitochondrial ribonucleotide supply by the nucleoside diphosphate kinase, NME6. Consequently, mt-dsRNA foci are profuse in cultured cancer cells and malignant cells of human tumour biopsies. Our results establish a new link between cell proliferation and mitochondrial nucleic acid homeostasis.
Identifiants
pubmed: 39209534
pii: 7/11/e202402764
doi: 10.26508/lsa.202402764
pmc: PMC11361371
pii:
doi:
Substances chimiques
RNA, Mitochondrial
0
RNA, Double-Stranded
0
DNA, Mitochondrial
0
Nucleoside-Diphosphate Kinase
EC 2.7.4.6
Banques de données
PDB
['2R20']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2024 Xavier et al.
Références
Oncotarget. 2016 May 3;7(18):26496-515
pubmed: 27034163
Nature. 2020 Dec;588(7839):712-716
pubmed: 33328633
Nat Struct Mol Biol. 2023 Oct;30(10):1549-1560
pubmed: 37679564
Nat Cell Biol. 2020 Oct;22(10):1180-1186
pubmed: 32989247
Cell Rep. 2022 Aug 9;40(6):111178
pubmed: 35947956
J Biol Chem. 2017 Mar 17;292(11):4519-4532
pubmed: 28082677
Nature. 2018 Aug;560(7717):238-242
pubmed: 30046113
Hepatology. 2020 Aug;72(2):609-625
pubmed: 31849082
Nat Rev Mol Cell Biol. 2022 Apr;23(4):286-301
pubmed: 34815573
Sci Rep. 2017 Sep 12;7(1):11257
pubmed: 28900194
Mol Biol Cell. 2003 Apr;14(4):1583-96
pubmed: 12686611
Mol Cell. 2018 Sep 20;71(6):1051-1063.e6
pubmed: 30174290
Nucleic Acids Res. 2013 Jan;41(2):1223-40
pubmed: 23221631
Nucleic Acids Res. 2020 Jun 4;48(10):5572-5590
pubmed: 32365187
EMBO Rep. 2017 Jan;18(1):28-38
pubmed: 27974379
J Biol Chem. 2009 Jul 31;284(31):20812-21
pubmed: 19509288
APMIS. 2023 May;131(5):197-205
pubmed: 36776120
EMBO J. 2023 Sep 18;42(18):e113256
pubmed: 37439264
Auto Immun Highlights. 2021 Mar 27;12(1):6
pubmed: 33773604
Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13534-9
pubmed: 21808029
Biol Chem. 2022 Mar 31;403(8-9):779-805
pubmed: 35355496
Cell Metab. 2013 Mar 5;17(3):386-98
pubmed: 23473033
Cell Metab. 2013 Mar 5;17(3):399-410
pubmed: 23473034
Nature. 1999 Jul 29;400(6743):464-8
pubmed: 10440377
Nucleic Acids Res. 2013 Mar 1;41(5):3068-78
pubmed: 23345615
Nucleic Acids Res. 2015 Sep 3;43(15):7398-413
pubmed: 26152302
J Inherit Metab Dis. 2022 Mar;45(2):292-307
pubmed: 35023579
Nat Commun. 2018 Jul 2;9(1):2558
pubmed: 29967381
J Cell Sci. 2009 Dec 15;122(Pt 24):4516-25
pubmed: 19920079
Curr Biol. 2022 Sep 12;32(17):3704-3719.e7
pubmed: 35896119
Mol Cell. 2023 Apr 6;83(7):1180-1196.e8
pubmed: 37028415
Nucleic Acids Res. 2010 Jan;38(1):279-98
pubmed: 19864255
J Virol. 2013 Jun;87(11):6314-25
pubmed: 23536668
Cell Rep. 2021 Nov 23;37(8):110000
pubmed: 34818548
Clin Transl Med. 2023 Sep;13(9):e1403
pubmed: 37670494
Nature. 2021 Mar;591(7850):477-481
pubmed: 33627873
Nat Commun. 2023 Mar 3;14(1):1223
pubmed: 36869030
Sci Signal. 2021 Aug 03;14(694):
pubmed: 34344832
Acta Histochem. 2017 Apr;119(3):315-326
pubmed: 28314612
Nucleic Acids Res. 2019 Apr 23;47(7):3680-3698
pubmed: 30715486
J Tissue Eng. 2010 Nov 07;2010:218142
pubmed: 21350642
Cell. 2015 Aug 27;162(5):961-73
pubmed: 26317465
Nat Cell Biol. 2023 Nov;25(11):1575-1589
pubmed: 37770567
Biochem Biophys Res Commun. 1975 Aug 18;65(4):1201-7
pubmed: 1052421
Nucleic Acids Res. 1991 Jun 11;19(11):2993-3000
pubmed: 2057357
Mol Cell. 2022 Oct 6;82(19):3712-3728.e10
pubmed: 36150385
Annu Rev Biochem. 2016 Jun 2;85:133-60
pubmed: 27023847
BMC Biol. 2004 May 24;2:9
pubmed: 15157274
Int J Mol Sci. 2021 Sep 01;22(17):
pubmed: 34502411
PLoS Pathog. 2016 Feb 10;12(2):e1005444
pubmed: 26862753
PLoS Genet. 2019 Jul 31;15(7):e1008240
pubmed: 31365523
Cell Metab. 2020 Sep 1;32(3):479-497.e9
pubmed: 32877691
Nat Rev Mol Cell Biol. 2024 Feb;25(2):119-132
pubmed: 37783784
Mol Ther Nucleic Acids. 2022 Sep 27;30:257-269
pubmed: 36284513
Sci Immunol. 2023 Oct 27;8(88):eadg2979
pubmed: 37862432
J Mol Biol. 1975 Dec 25;99(4):809-14
pubmed: 1214305
J Biol Chem. 2008 Oct 3;283(40):27064-73
pubmed: 18678873
Nature. 2023 Mar;615(7952):490-498
pubmed: 36890227
J Virol. 2006 May;80(10):5059-64
pubmed: 16641297
Cell. 2015 Aug 27;162(5):974-86
pubmed: 26317466
STAR Protoc. 2023 Mar 17;4(1):102007
pubmed: 36853732