Generation of rat offspring from ovarian oocytes by xenotransplantation.

Animals Female Oocytes Transplantation, Heterologous / methods Rats Mice Ovary / transplantation Fertilization in Vitro / methods
Embryo Immunodeficient mouse Offspring production Ovary Rat Xenotransplantation

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
29 Aug 2024
Historique:
received: 08 03 2024
accepted: 23 08 2024
medline: 31 8 2024
pubmed: 31 8 2024
entrez: 29 8 2024
Statut: epublish

Résumé

The idea of utilizing unused oocytes present in the ovaries has been tested in various ways to produce offspring. However, only a limited number of studies succeeded in offspring generation. They include transplantation of ovaries into autologous or allogeneic animals, and acquisition of pups from oocytes obtained by transplanting mouse ovaries into immunodeficient rats. Here we report successful production of rat oocytes by transplanting rat ovaries under the kidney capsule of immunodeficient mice with addition of hormone administration to the mice. In addition, these oocytes were developed by in vitro fertilization, and transplanted into the oviducts of pseudopregnant rats, resulting in successful delivery of pups. The modified gene of the donor rat was confirmed to be correctly inherited to the pups. These results show that xenotransplantation of ovarian tissue makes it possible to leave offspring, beginning a new phase in developmental engineering.

Identifiants

DOI: 10.1038/s41598-024-71030-0 PMID: 39209914
pubmed: 39209914
doi: 10.1038/s41598-024-71030-0
pii: 10.1038/s41598-024-71030-0
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

20109

Subventions

Organisme : JST SPRING Grant
ID : JPMJSP2145
Organisme : Japan Agency for Medical Research and Development
ID : JP23dm0207091h0005
Organisme : Japan Society for the Promotion of Science
ID : JP16H06276

Informations de copyright

© 2024. The Author(s).

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Auteurs

Hiroaki Taketsuru (H)

Department of Animal Model Development, Brain Research Institute, Niigata University, Niigata, 951-8585, Japan.
Department of Comparative and Experimental Medicine, Brain Research Institute, Niigata University, Niigata, 951-8585, Japan.
Division of Animal Genetics, Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.

Runa Hirayama (R)

Department of Animal Model Development, Brain Research Institute, Niigata University, Niigata, 951-8585, Japan.
Department of Behavioral Physiology, Graduate School of Innovative Life Science, University of Toyama, Toyama, 930-0194, Japan.

Ena Nakatsukasa (E)

Department of Animal Model Development, Brain Research Institute, Niigata University, Niigata, 951-8585, Japan.

Rie Natsume (R)

Department of Animal Model Development, Brain Research Institute, Niigata University, Niigata, 951-8585, Japan.

Keizo Takao (K)

Department of Behavioral Physiology, Graduate School of Innovative Life Science, University of Toyama, Toyama, 930-0194, Japan.
Department of Behavioral Physiology, Faculty of Medicine, University of Toyama, Toyama, 930-0194, Japan.
Research Center for Idling Brain Science, University of Toyama, Toyama, 930-0194, Japan.

Manabu Abe (M)

Department of Animal Model Development, Brain Research Institute, Niigata University, Niigata, 951-8585, Japan. manabu@bri.niigata-u.ac.jp.

Kenji Sakimura (K)

Department of Animal Model Development, Brain Research Institute, Niigata University, Niigata, 951-8585, Japan. sakimura@bri.niigata-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Generation of rat offspring from ovarian...
questionsmedicales.fr › Cellules › Cellules germinales › Ovocytes › Generation of rat offspring from ovarian...
questionsmedicales.fr › Procédures de chirurgie opératoire › Transplantation › Transplantation hétérologue › Generation of rat offspring from ovarian...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Generation of rat offspring from ovarian...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Generation of rat offspring from ovarian...
questionsmedicales.fr › Système endocrine › Glandes endocrines › Gonades › Ovaire › Generation of rat offspring from ovarian...
questionsmedicales.fr › Techniques d'investigation › Techniques de reproduction › Techniques de reproduction assistée › Fécondation in vitro › Generation of rat offspring from ovarian...