Reliability of In Vivo Creatine-Weighted Chemical Exchange Saturation Transfer (CrCEST) MRI in Calf Skeletal Muscle of Healthy Volunteers at 3 T.
CrCEST reliability
OXPHOS
interobserver reproducibility
interscan repeatability
intraobserver reproducibility
plantar flexion
Journal
Journal of magnetic resonance imaging : JMRI
ISSN: 1522-2586
Titre abrégé: J Magn Reson Imaging
Pays: United States
ID NLM: 9105850
Informations de publication
Date de publication:
30 Aug 2024
30 Aug 2024
Historique:
revised:
29
07
2024
received:
01
05
2024
accepted:
29
07
2024
medline:
31
8
2024
pubmed:
31
8
2024
entrez:
30
8
2024
Statut:
aheadofprint
Résumé
Skeletal muscle mitochondrial oxidative phosphorylation (mtOXPHOS) is important for ATP generation and its dysfunction leads to exercise intolerance. Phosphorus magnetic resonance spectroscopy ( To evaluate the interscan repeatability, intra- and interobserver reproducibility of CrCEST during mild plantar flexion exercise. Retrospective. Twenty healthy volunteers (age 37.6 ± 12.4 years, 11 females). 3 T/CEST imaging using gradient echo readout. τCrCEST (postexercise Cr recovery time) was assessed in two scans for each participant, following mild plantar flexion exercises targeting the medial gastrocnemius (MG), lateral gastrocnemius (LG), and soleus (Sol) muscles. Three observers measured τCrCEST for interobserver reproducibility. Three readings by one observer were used to measure intraobserver reproducibility. Two scans were used for within-participant interscan repeatability. Paired t tests, intraclass correlation coefficient (ICC), and Pearson correlation were conducted. Bland-Altman plots were used to analyze the interobserver variability. A P-value of 0.05 was considered statistically significant. There was excellent intra- (ICC CrCEST MRI shows promise in assessing muscle bioenergetics by evaluating τCrCEST during mild plantar flexion exercise with reasonable reliability, particularly in LG and MG. 4 TECHNICAL EFFICACY: Stage 1.
Sections du résumé
BACKGROUND
BACKGROUND
Skeletal muscle mitochondrial oxidative phosphorylation (mtOXPHOS) is important for ATP generation and its dysfunction leads to exercise intolerance. Phosphorus magnetic resonance spectroscopy (
PURPOSE
OBJECTIVE
To evaluate the interscan repeatability, intra- and interobserver reproducibility of CrCEST during mild plantar flexion exercise.
STUDY TYPE
METHODS
Retrospective.
SUBJECTS
METHODS
Twenty healthy volunteers (age 37.6 ± 12.4 years, 11 females).
FIELD STRENGTH/SEQUENCE
UNASSIGNED
3 T/CEST imaging using gradient echo readout.
ASSESSMENT
RESULTS
τCrCEST (postexercise Cr recovery time) was assessed in two scans for each participant, following mild plantar flexion exercises targeting the medial gastrocnemius (MG), lateral gastrocnemius (LG), and soleus (Sol) muscles. Three observers measured τCrCEST for interobserver reproducibility. Three readings by one observer were used to measure intraobserver reproducibility. Two scans were used for within-participant interscan repeatability.
STATISTICAL TESTS
METHODS
Paired t tests, intraclass correlation coefficient (ICC), and Pearson correlation were conducted. Bland-Altman plots were used to analyze the interobserver variability. A P-value of 0.05 was considered statistically significant.
RESULTS
RESULTS
There was excellent intra- (ICC
DATA CONCLUSION
CONCLUSIONS
CrCEST MRI shows promise in assessing muscle bioenergetics by evaluating τCrCEST during mild plantar flexion exercise with reasonable reliability, particularly in LG and MG.
LEVEL OF EVIDENCE
METHODS
4 TECHNICAL EFFICACY: Stage 1.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : St. Jude Children's Research Hospital, Comprehensive Cancer Center Grant
Organisme : National Institue on Aging
ID : R01-AG071725
Organisme : NCI NIH HHS
ID : R25CA23944
Pays : United States
Organisme : American Lebanese Syrian Associated Charities (ALSAC)
Informations de copyright
© 2024 International Society for Magnetic Resonance in Medicine.
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