Leishmania major telomerase RNA knockout: From altered cell proliferation to decreased parasite infectivity.

Altered cell proliferation Decreased infectivity capacity Leishmania spp. TERRA upregulation Telomerase RNA knockout and overexpression Telomere shortening

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
30 Aug 2024
Historique:
received: 07 06 2024
revised: 14 08 2024
accepted: 27 08 2024
medline: 2 9 2024
pubmed: 2 9 2024
entrez: 1 9 2024
Statut: aheadofprint

Résumé

This study focuses on the biological impacts of deleting the telomerase RNA from Leishmania major (LeishTER), a parasite responsible for causing leishmaniases, for which no effective treatment or prevention is available. TER is a critical player in the telomerase ribonucleoprotein complex, containing the template sequence copied by the reverse transcriptase component during telomere elongation. The success of knocking out both LeishTER alleles was confirmed, and no off-targets were detected. LmTER

Identifiants

DOI: 10.1016/j.ijbiomac.2024.135150 PMID: 39218181
pubmed: 39218181
pii: S0141-8130(24)05956-7
doi: 10.1016/j.ijbiomac.2024.135150
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

135150

Informations de copyright

Copyright © 2024. Published by Elsevier B.V.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors confirm that no financial or other relationship might lead to any potential competing interests.

Auteurs

Beatriz Cristina Dias de Oliveira (BCD)

Department of Chemical and Biological Sciences, Biosciences Institute, São Paulo State University (UNESP), Botucatu, Sao Paulo, Brazil.

Mark Ewusi Shiburah (ME)

Department of Chemical and Biological Sciences, Biosciences Institute, São Paulo State University (UNESP), Botucatu, Sao Paulo, Brazil; Animal Research Institute, Council for Scientific and Industrial Research (CSIR-ARI), Accra, Ghana.

Luiz Henrique Castro Assis (LHC)

Department of Chemical and Biological Sciences, Biosciences Institute, São Paulo State University (UNESP), Botucatu, Sao Paulo, Brazil.

Veronica Silva Fontes (VS)

Department of Chemical and Biological Sciences, Biosciences Institute, São Paulo State University (UNESP), Botucatu, Sao Paulo, Brazil.

Habtye Bisetegn (H)

Department of Chemical and Biological Sciences, Biosciences Institute, São Paulo State University (UNESP), Botucatu, Sao Paulo, Brazil; Department of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia.

Arthur de Oliveira Passos (A)

Department of Chemical and Biological Sciences, Biosciences Institute, São Paulo State University (UNESP), Botucatu, Sao Paulo, Brazil.

Leilane S de Oliveira (LS)

Department of Chemical and Biological Sciences, Biosciences Institute, São Paulo State University (UNESP), Botucatu, Sao Paulo, Brazil.

Cristiane de Santis Alves (C)

Howard Hughes Medical Institute/Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.

Evan Ernst (E)

Howard Hughes Medical Institute/Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.

Rob Martienssen (R)

Howard Hughes Medical Institute/Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.

Pedro Henrique Gallo-Francisco (PH)

Department of Animal Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil.

Selma Giorgio (S)

Department of Animal Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil.

Marcos Meuser Batista (MM)

Cellular Biology Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brazil.

Maria de Nazaré Correia Soeiro (M)

Cellular Biology Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brazil.

Rubem Figueiredo Sadok Menna-Barreto (RFS)

Cellular Biology Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brazil.

Juliana Ide Aoki (JI)

Department of Animal Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil.

Adriano Cappellazzo Coelho (AC)

Department of Animal Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil.

Maria Isabel Nogueira Cano (MIN)

Department of Chemical and Biological Sciences, Biosciences Institute, São Paulo State University (UNESP), Botucatu, Sao Paulo, Brazil. Electronic address: maria.in.cano@unesp.br.

Classifications MeSH