A guide to selecting high-performing antibodies for Protein-glutamine gamma-glutamyltransferase 2 (TGM2) for use in western blot, immunoprecipitation and immunofluorescence.

Humans Transglutaminases / immunology Blotting, Western Fluorescent Antibody Technique / methods Protein Glutamine gamma Glutamyltransferase 2 Immunoprecipitation / methods Antibodies / immunology GTP-Binding Proteins / immunology
Protein-glutamine gamma-glutamyltransferase 2 TGM TGM2 Uniprot ID P21980 antibody characterization antibody validation immunofluorescence immunoprecipitation western blot

Journal

F1000Research
ISSN: 2046-1402
Titre abrégé: F1000Res
Pays: England
ID NLM: 101594320

Informations de publication

Date de publication:
2024
Historique:
accepted: 25 07 2024
medline: 2 9 2024
pubmed: 2 9 2024
entrez: 2 9 2024
Statut: epublish

Résumé

Protein-glutamine gamma-glutamyltransferase 2 (TGM2) is a Ca

Identifiants

DOI: 10.12688/f1000research.150684.2 PMID: 39220380 PMC: PMC11362715
pubmed: 39220380
doi: 10.12688/f1000research.150684.2
pmc: PMC11362715
doi:

Substances chimiques

Transglutaminases EC 2.3.2.13
Protein Glutamine gamma Glutamyltransferase 2 EC 2.3.2.13
Antibodies 0
GTP-Binding Proteins EC 3.6.1.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

481

Informations de copyright

Copyright: © 2024 Ayoubi R et al.

Déclaration de conflit d'intérêts

Competing interests: For this project, the laboratory of Peter McPherson developed partnerships with high-quality antibody manufacturers and knockout cell line providers. The partners provide antibodies and knockout cell lines to the McPherson laboratory at no cost. These partners include: - ABCD antibodies - Abcam - Aviva Systems Biology -Bio Techne -Cell Signalling Technology -Developmental Studies Hybridoma Bank -GeneTex – Horizon Discovery – Proteintech – Synaptic Systems –Thermo Fisher Scientific.

Auteurs

Riham Ayoubi (R)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.

Maryam Fotouhi (M)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.

Charles Alende (C)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
ORCID: 0009-0005-4611-6134

Sara González Bolívar (S)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
ORCID: 0000-0002-4299-8281

Kathleen Southern (K)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
ORCID: 0000-0002-4125-3608

Carl Laflamme (C)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
ORCID: 0000-0001-5906-025X

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A guide to selecting high-performing...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Acyltransferases › Aminoacyltransferases › Transglutaminases › A guide to selecting high-performing...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Immunotransfert › Technique de Western › A guide to selecting high-performing...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Technique d'immunofluorescence › A guide to selecting high-performing...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Acyltransferases › Aminoacyltransferases › Transglutaminases › Protein glutamine gamma glutamyltransferase-2 › A guide to selecting high-performing...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Immunoprécipitation › A guide to selecting high-performing...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › A guide to selecting high-performing...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines G › A guide to selecting high-performing...