Seasonal Prevalence and Detection of Enteric and Respiratory Viruses in Wastewater and Hospitalized Children with Acute Gastroenteritis.
Humans
Gastroenteritis
/ virology
Wastewater
/ virology
Seasons
Prevalence
Sewage
/ virology
Child
Feces
/ virology
Child, Preschool
Child, Hospitalized
Infant
SARS-CoV-2
/ genetics
Viruses
/ isolation & purification
Kobuvirus
/ isolation & purification
Human bocavirus
/ isolation & purification
Male
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
02 Sep 2024
02 Sep 2024
Historique:
received:
27
05
2024
accepted:
11
08
2024
medline:
3
9
2024
pubmed:
3
9
2024
entrez:
2
9
2024
Statut:
epublish
Résumé
Human enteric and some respiratory viruses are identified to be involved with acute gastroenteritis that can be shed in feces of infected persons into the environment. Understanding the abundance of these viruses in wastewater is significant when assessing the public health impacts associated with discharge of wastewater into the environment. This study aimed to investigate the prevalence and seasonal variation of human enteric adenovirus (HAdV), Aichi virus (AiV-1), bocavirus (HBoV), and respiratory syndrome coronavirus 2 virus (SARS-CoV-2) in wastewater as well as their prevalence among hospitalized children with acute gastroenteritis. The viruses were detected and quantified with real-time PCR. HAdV was the most detected virus in raw sewage (88.5%), treated sewage (28%), and stool gastroenteritis (74%), followed by HBoV (45.8% for raw sewage, 14.6% for treated sewage, and 55.3% for stool samples). The detection rate of AiV-1 was 59.4%, 19.8%, and 62.6% in raw sewage, treated sewage, and stool samples, respectively. The rate of SARS-CoV-2 detection in raw sewage, treated sewage, and stool samples was 33.3%, 7.3%, and 20.6%, respectively. The viral concentrations ranged between 4.50 × 10
Identifiants
pubmed: 39223408
doi: 10.1007/s00284-024-03841-3
pii: 10.1007/s00284-024-03841-3
doi:
Substances chimiques
Wastewater
0
Sewage
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
337Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Bhutta ZA, Sommerfeld J, Lassi ZS, Salam RA, Das JK (2014) Global burden, distribution, and interventions for infectious diseases of poverty. Infect Dis Poverty 3:1–7. https://doi.org/10.1186/2049-9957-3-21
doi: 10.1186/2049-9957-3-21
Lafferty KD (2009) The ecology of climate change and infectious diseases. Ecology 90:888–900. https://doi.org/10.1890/08-0079.1
doi: 10.1890/08-0079.1
pubmed: 19449681
Manetu WM, M’masi S, Recha CW, (2021) Diarrhea disease among children under 5 years of age: a global systematic review. Open J Epidemiol 11(3):207–221. https://doi.org/10.4236/ojepi.2021.113018
doi: 10.4236/ojepi.2021.113018
Shaheen MN, Abd Al-Daim SE, Ahmed NI, Khalifa MK, Seif WH, Ahmed SS et al (2024) Prevalence and molecular characterization of rotavirus strains circulating among children with gastroenteritis in Egypt. Egypt Pharm J 23(2):223–236. https://doi.org/10.4103/epj.epj_326_23
doi: 10.4103/epj.epj_326_23
Bozkurt D, Selimoğlu MA, Otlu B, Sandıkkaya A (2015) Eight different viral agents in childhood acute gastroenteritis. Turkish J Pediatr 57(1):68–73
Lanrewaju AA, Enitan-Folami AM, Sabiu S, Edokpayi JN, Swalaha FM (2022) Global public health implications of human exposure to viral contaminated water. Front Microbiol 13:981896. https://doi.org/10.3389/fmicb.2022.981896
doi: 10.3389/fmicb.2022.981896
pubmed: 36110296
pmcid: 9468673
Shrestha S, Yoshinaga E, Chapagain SK, Mohan G, Gasparatos A, Fukushi K (2021) Wastewater-based epidemiology for cost-effective mass surveillance of COVID-19 in low-and middle-income countries: challenges and opportunities. Water 13(20):2897. https://doi.org/10.3390/w13202897
doi: 10.3390/w13202897
Hellmér M, Paxéus N, Magnius L, Enache L, Arnholm B, Johansson A et al (2014) Detection of pathogenic viruses in sewage provided early warnings of hepatitis a virus and norovirus outbreaks. Appl Environ Microbiol 80(21):6771–6781. https://doi.org/10.1128/AEM.01981-14
doi: 10.1128/AEM.01981-14
pubmed: 25172863
pmcid: 4249052
Shaheen MN, Ahmed N, Rady BK, Elmahdy EM (2024) Detection and quantification of adenovirus, polyomavirus, and papillomavirus in urban sewage. J Water Health 22(2):401–413. https://doi.org/10.2166/wh.2024.322
doi: 10.2166/wh.2024.322
pubmed: 38421633
Shaheen MNF, Elmahdy EM, Shahein YE (2022) The first detection of SARS-CoV-2 RNA in urban wastewater in Giza. Egypt J Water Health 20(8):1212–1222. https://doi.org/10.2166/wh.2022.098
doi: 10.2166/wh.2022.098
pubmed: 36044190
Wolfe MK, Yu AT, Duong D, Rane MS, Hughes B, Chan-Herur V et al (2023) Use of wastewater for Mpox outbreak surveillance in california. N Engl J Med. https://doi.org/10.1056/NEJMc2213882
doi: 10.1056/NEJMc2213882
pubmed: 36652340
Stobnicka-Kupiec A, Gołofit-Szymczak M, Cyprowski M, Górny RL (2022) Detection and identification of potentially infectious gastrointestinal and respiratory viruses at workplaces of wastewater treatment plants with viability qPCR/RT-qPCR. Sci Rep 12(1):4517. https://doi.org/10.1038/s41598-022-08452-1
doi: 10.1038/s41598-022-08452-1
pubmed: 35296727
pmcid: 8924946
Wu X, Zhang J, Lan W, Quan L, Ou J, Zhao W et al (2022) Molecular typing and rapid identification of human adenoviruses associated with respiratory diseases using universal PCR and sequencing primers for the three major capsid genes: penton base, Hexon, and Fiber. Front Microbiol 13:911694. https://doi.org/10.3389/fmicb.2022.911694
doi: 10.3389/fmicb.2022.911694
pubmed: 35633710
pmcid: 9133664
Öner SZ, Kaleli O, Demir M, Mete E, Çalişkan A (2022) Rotavirus and adenovirus prevalence in patients with acute viral gastroenteritis in denizli, turkey, 2017–2021. J Med Virol 94(8):3857–3862. https://doi.org/10.1002/jmv.27834
doi: 10.1002/jmv.27834
pubmed: 35510351
Kujawski SA, Lu X, Schneider E, Blythe D, Boktor S, Farrehi J et al (2021) Outbreaks of adenovirus associated respiratory illness on 5 college campuses in the united states, 2018–2019. Clin Infect Dis. 72(11):19–99
doi: 10.1093/cid/ciaa465
Jarecki-Khan K, Tzipori SR, Unicomb LE (1993) Enteric adenovirus infection among infants with diarrhea in rural Bangladesh. J Clin Microbiol 31(3):484–489. https://doi.org/10.1128/jcm.31.3.484-489.1993
doi: 10.1128/jcm.31.3.484-489.1993
pubmed: 8458940
pmcid: 262806
Dias J, Pinto RN, Vieira CB, de Abreu CA (2018) Detection and quantification of human adenovirus (HAdV), JC polyomavirus (JCPyV) and hepatitis A virus (HAV) in recreational waters of Niterói, Rio de Janeiro, Brazil. Mar Pollut Bull 133:240–245. https://doi.org/10.1016/j.marpolbul.2018.05.031
doi: 10.1016/j.marpolbul.2018.05.031
pubmed: 30041311
Yamashita T, Sugiyama M, Tsuzuki H, Sakae K, Suzuki Y, Miyazaki Y (2000) Application of a reverse transcription-PCR for identification and differentiation of Aichi virus, a new member of the picornavirus family associated with gastroenteritis in humans. J Clin Microbiol 38(8):2955–2961. https://doi.org/10.1128/JCM.38.8.2955-2961.2000
doi: 10.1128/JCM.38.8.2955-2961.2000
pubmed: 10921958
pmcid: 87158
Kitajima M, Haramoto E, Phanuwan C, Katayama H (2011) Prevalence and genetic diversity of aichi viruses in wastewater and river water in Japan. Appl Environ Microbiol 77(6):2184–2187. https://doi.org/10.1128/AEM.02328-10
doi: 10.1128/AEM.02328-10
pubmed: 21257803
pmcid: 3067340
Yamashita T, Ito M, Tsuzuki H, Sakae K (2001) Identification of Aichi virus infection by measurement of immunoglobulin responses in an enzyme-linked immunosorbent assay. J Clin Microbiol. https://doi.org/10.1128/JCM.39.11.4178-4180.2001
doi: 10.1128/JCM.39.11.4178-4180.2001
pubmed: 11682554
pmcid: 88511
Haramoto E, Kitajima M (2017) Quantification and genotyping of Aichi virus 1 in water samples in the kathmandu valley. Nepal Food Environ Virol 9:350–353. https://doi.org/10.1007/s12560-017-9283-7
doi: 10.1007/s12560-017-9283-7
pubmed: 28185207
Onosi O, Upfold NS, Jukes MD, Luke GA, Knox C (2019) The first molecular detection of Aichi virus 1 in raw sewage and mussels collected in South Africa. Food Environ Virol 11(1):96–100. https://doi.org/10.1007/s12560-018-9362-4
doi: 10.1007/s12560-018-9362-4
pubmed: 30560489
Jartti T, Hedman K, Jartti L, Ruuskanen O, Allander T, Söderlund-Venermo M (2012) Human bocavirus—the first 5 years. Rev Med Virol 22(1):46–64. https://doi.org/10.1002/rmv.720
doi: 10.1002/rmv.720
pubmed: 22038931
De R, Liu L, Qian Y, Zhu R, Deng J, Wang F et al (2017) Risk of acute gastroenteritis associated with human bocavirus infection in children: a systematic review and meta-analysis. PLoS ONE 12(9):e0184833. https://doi.org/10.1371/journal.pone.0184833
doi: 10.1371/journal.pone.0184833
pubmed: 28910409
pmcid: 5599015
Jiang L, Li X, Nie J, Tang K, Bhutta ZA (2023) A systematic review of persistent clinical features after SARS-CoV-2 in the pediatric population. Pediatrics. https://doi.org/10.1542/peds.2022-060351
doi: 10.1542/peds.2022-060351
pubmed: 37872474
pmcid: 10507889
Zhang H, Weng Z, Zheng Y, Zheng M, Chen W, He H et al (2023) Epidemiological and clinical features of SARS-CoV-2 omicron variant infection in Quanzhou, Fujian province: a retrospective study. Sci Rep 13(1):22152. https://doi.org/10.1038/s41598-023-49098-x
doi: 10.1038/s41598-023-49098-x
pubmed: 38092887
pmcid: 10719353
Buonsenso D, Ferro V, Viozzi F, Morello R, Proli F, Bersani G et al (2024) Changes in clinical, demographic, and outcome patterns of children hospitalized with non-SARS-CoV-2 viral low respiratory tract infections before and during the COVID pandemic in rome. Italy Pediat Pulmonol 59(2):362–370. https://doi.org/10.1002/ppul.26755 . (Epub 2023 Nov 8)
doi: 10.1002/ppul.26755
Chen Y, Chen L, Deng Q, Zhang G, Wu K, Ni L et al (2020) The presence of SARS-CoV-2 RNA in the feces of COVID-19 patients. J Med Virol 92(7):833–840. https://doi.org/10.1002/jmv.25825
doi: 10.1002/jmv.25825
pubmed: 32243607
Haramoto E, Malla B, Thakali O, Kitajima M (2020) First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan. Sci Total Environ 737:140405. https://doi.org/10.1016/j.scitotenv.2020.140405
doi: 10.1016/j.scitotenv.2020.140405
pubmed: 32783878
pmcid: 7305903
Sapula SA, Whittall JJ, Pandopulos AJ, Gerber C, Venter H (2021) An optimized and robust PEG precipitation method for detection of SARS-CoV-2 in wastewater. Sci Total Environ 785:147270. https://doi.org/10.1016/j.scitotenv.2021.147270
doi: 10.1016/j.scitotenv.2021.147270
pubmed: 33940413
pmcid: 8086323
Booranathawornsom T, Pombubpa K, Tipayamongkholgul M, Kittigul L (2022) Molecular characterization of human bocavirus in recycled water and sewage sludge in Thailand. Infect Genet Evol 100:105276. https://doi.org/10.1007/s12560-020-09445-x
doi: 10.1007/s12560-020-09445-x
pubmed: 35367361
Lu X, Wang L, Sakthivel SK, Whitaker B, Murray J, Kamili S et al (2020) US CDC real-time reverse transcription PCR panel for detection of severe acute respiratory syndrome coronavirus 2. Emerg Infect Dis 26(8):1654. https://doi.org/10.3201/eid2608.201246
doi: 10.3201/eid2608.201246
pubmed: 32396505
pmcid: 7392423
DongY KJ, Lewis GD (2010) Evaluation of methodology for detection of human adenoviruses in wastewater, drinking water, stream water and recreational waters. J Appl Microbiol 108(3):800–809. https://doi.org/10.1111/j.1365-2672.2009.04477.x
doi: 10.1111/j.1365-2672.2009.04477.x
La Rosa G, Della Libera S, Iaconelli M, Donia D, Cenko F, Xhelilaj G et al (2016) Human bocavirus in children with acute gastroenteritis in Albania. J Med Virol 88(5):906–910. https://doi.org/10.1002/jmv.24415
doi: 10.1002/jmv.24415
pubmed: 26496439
Castiglioni S, Schiarea S, Pellegrinelli L, Primache V, Galli C, Bubba L et al (2022) SARS-CoV-2 RNA in urban wastewater samples to monitor the COVID-19 pandemic in lombardy, Italy (March–June 2020). Sci Total Environ 806:150816. https://doi.org/10.1016/j.scitotenv.2021.150816
doi: 10.1016/j.scitotenv.2021.150816
pubmed: 34627901
Ahmed W, Angel N, Edson J, Bibby K, Bivins A, O’Brien JW et al (2020) First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: a proof of concept for the wastewater surveillance of COVID-19 in the community. Sci Total Environ 728:138764. https://doi.org/10.1016/j.scitotenv.2020.138764
doi: 10.1016/j.scitotenv.2020.138764
pubmed: 32387778
pmcid: 7165106
Kumar M, Patel AK, Shah AV, Raval J, Rajpara N, Joshi M et al (2020) First proof of the capability of wastewater surveillance for COVID-19 in India through detection of genetic material of SARS-CoV-2. Sci Total Environ 746:141326. https://doi.org/10.1016/j.scitotenv.2020.141326
doi: 10.1016/j.scitotenv.2020.141326
pubmed: 32768790
pmcid: 7386605
Randazzo W, Truchado P, Cuevas-Ferrando E, Simón P, Allende A, Sánchez G (2020) SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area. Water Res 181:115942. https://doi.org/10.1016/j.watres.2020.115942
doi: 10.1016/j.watres.2020.115942
pubmed: 32425251
pmcid: 7229723
Rattanakul S, Oguma K, Sakai H, Takizawa S (2014) Inactivation of viruses by combination processes of UV and chlorine. J Water Environ Technol 12(6):511–523. https://doi.org/10.2965/jwet.2014.511
doi: 10.2965/jwet.2014.511
Elmahdy EM, Ahmed NI, Shaheen MN, Mohamed ECB, Loutfy SA (2019) Molecular detection of human adenovirus in urban wastewater in Egypt and among children suffering from acute gastroenteritis. J Water Health 17(2):287–294. https://doi.org/10.2166/wh.2019.303
doi: 10.2166/wh.2019.303
pubmed: 30942778
Schlindwein AD, Rigotto C, Simões CMO, Barardi CRM (2010) Detection of enteric viruses in sewage sludge and treated wastewater effluent. Water Sci Technol 61(2):537–544. https://doi.org/10.2166/wst.2010.845
doi: 10.2166/wst.2010.845
pubmed: 20107281
Badr KR, Elmahdy EM, Shaheen MN (2024) Molecular detection of Human adenovirus and human bocavirus in wastewater and drain water in the western nile delta of egypt. Water Supply 24(2):453. https://doi.org/10.2166/ws.2024.001
doi: 10.2166/ws.2024.001
Shaheen MNF, Abd El-Daim SE, Ahmed NI, Elmahdy EM (2020) Environmental monitoring of Aichi virus and human bocavirus in samples from wastewater treatment plant, drain, and River Nile in Egypt. J Water Health 18(1):30–37. https://doi.org/10.2166/wh.2019.075
doi: 10.2166/wh.2019.075
pubmed: 32129184
Burutaran L, Lizasoain A, Garcia M, Tort LF, Colina R, Victoria M (2016) Detection and molecular characterization of aichivirus 1 in wastewater samples from uruguay. Food Environ Virol 8(1):13–17. https://doi.org/10.1007/s12560-015-9217-1
doi: 10.1007/s12560-015-9217-1
pubmed: 26456918
Kumthip K, Khamrin P, Yodmeeklin A, Maneekarn N (2020) Prevalence and genetic characterization of aichivirus in environmental waters in Thailand. Food Environ Virol 12:342–349. https://doi.org/10.1007/s12560-020-09445-x
doi: 10.1007/s12560-020-09445-x
pubmed: 33044663
Toribio-Avedillo D, Gómez-Gómez C, Sala-Comorera L, Rodríguez-Rubio L, Carcereny A, García-Pedemonte D et al (2023) Monitoring influenza and respiratory syncytial virus in wastewater, beyond COVID-19. Sci Total Environ 892:164495. https://doi.org/10.1016/j.scitotenv.2023.164495
doi: 10.1016/j.scitotenv.2023.164495
pubmed: 37245831
pmcid: 10214770
La Rosa G, Pourshaban M, Iaconelli M, Muscillo M (2010) Quantitative real-time PCR of enteric viruses in influent and effluent samples from wastewater treatment plants in Italy. Annali dell’Istituto superiore di sanita 46:266–273. https://doi.org/10.4415/ANN_10_03_07
doi: 10.4415/ANN_10_03_07
pubmed: 20847459
Victoria M, Guimaraes FR, Fumian TM, Ferreira FF, Vieira CB, Shubo T et al (2010) One year monitoring of norovirus in a sewage treatment plant in Rio de Janeiro, Brazil. J Water Health 8:158–165. https://doi.org/10.2166/wh.2009.012
doi: 10.2166/wh.2009.012
pubmed: 20009258
Chuchaona W, Khamrin P, Yodmeeklin A, Kumthip K, Saikruang W, Thongprachum A et al (2017) Detection and characterization of Aichi virus 1 in pediatric patients with diarrhea in Thailand. J Med Virol 89(2):234–238. https://doi.org/10.1002/jmv.24630
doi: 10.1002/jmv.24630
pubmed: 27420653
Bouazizi A, Fredj MBH, Bennour H, Jerbi A, Fodha I, Trabelsi A (2024) Molecular analysis of adenovirus strains responsible for gastroenteritis in children, under five, in Tunisia. Heliyon. https://doi.org/10.1016/j.heliyon.2023.e22969
doi: 10.1016/j.heliyon.2023.e22969
pubmed: 38163238
Fumian TM, Malta FC, Dos Santos DR, Pauvolid-Corrêa A, Fialho AM, Leite JP et al (2021) SARS-CoV-2 RNA detection in stool samples from acute gastroenteritis cases. Brazil J Med Virol 93(4):2543–2547. https://doi.org/10.1002/jmv.26786
doi: 10.1002/jmv.26786
pubmed: 33421163
Rivadulla E, Varela MF, Romalde JL (2019) Epidemiology of Aichi virus in fecal samples from outpatients with acute gastroenteritis in northwestern spain. J Clin Virol 118:14–19. https://doi.org/10.1016/j.jcv.2019.07.011
doi: 10.1016/j.jcv.2019.07.011
pubmed: 31382225
Ndjangangoye NK, Lekana-Douki SE, Lekolo GM, Mve-Ella OB, Oyegue-Liabagui SL, Lekana-Douki JB (2021) High prevalence and prolonged shedding with enteric viruses among children with acute diarrhea in franceville, southeast of Gabon. J Clin Virol Plus 1(4):100046. https://doi.org/10.1016/j.jcvp.2021.100046
doi: 10.1016/j.jcvp.2021.100046
Liguoro I, Pilotto C, Bonanni M, Ferrari ME, Pusiol A, Nocerino A et al (2020) SARS-COV-2 infection in children and newborns: a systematic review. Eur J Pediatr 179:1029–1046. https://doi.org/10.1007/s00431-020-03684-7
doi: 10.1007/s00431-020-03684-7
pubmed: 32424745
pmcid: 7234446
Chacón L, Morales E, Valiente C, Reyes L, Barrantes K (2021) Wastewater-based epidemiology of enteric viruses and surveillance of acute gastrointestinal illness outbreaks in a resource-limited region. Am J Trop Med Hyg 105(4):1004. https://doi.org/10.4269/ajtmh.21-0050
doi: 10.4269/ajtmh.21-0050
pubmed: 34339385
pmcid: 8592140
Taghinejad M, Mostafa G, Seyed DM (2020) High frequency of aichivirus with acute gastroenteritis in iran. Pediatr Infect Dis J 39(7):576–579. https://doi.org/10.1097/INF.0000000000002638
doi: 10.1097/INF.0000000000002638
pubmed: 32097244
Kitajima M, Hata A, Yamashita T, Haramoto EH, Katayama H (2013) Development of a reverse transcription- quantitative PCR system for detection and genotyping of aichi viruses in clinical and environmental samples. Appl Environ Microbiol 79:3952–3958. https://doi.org/10.1128/AEM.00820-13
doi: 10.1128/AEM.00820-13
pubmed: 23603673
pmcid: 3697579
Zhao B, Yu X, Wang C, Teng Z, Wang C, Shen J et al (2013) High human bocavirus viral load is associated with disease severity in children under five years of age. PLoS ONE 8(4):e62318. https://doi.org/10.1371/journal.pone.0062318
doi: 10.1371/journal.pone.0062318
pubmed: 23638038
pmcid: 3640090
Kantola K, Sadeghi M, Antikainen J, Kirveskari J, Delwar E, Hedman K et al (2010) Real-time quantitative PCR detection of four human bocaviruses. J Clin Microbiol 48(11):4044–4050. https://doi.org/10.1128/JCM.00686-10
doi: 10.1128/JCM.00686-10
pubmed: 20844210
pmcid: 3020864
Vetter MR, Staggemeier R, Vecchia AD, Henzel A, Rigotto C, Spilki FR (2015) Seasonal variation on the presence of adenoviruses in stools from non-diarrheic patients. Braz J Microbiol 46:749–752. https://doi.org/10.1590/S1517-838246320140718
doi: 10.1590/S1517-838246320140718
pubmed: 26413056
pmcid: 4568858
Mohammad MY, Al-Shuwaikh AM, Al-Hamdani HT (2023) Evaluation of human bocavirus (HBoV) as a cause of acute gastroenteritis in children under 5 years: a case-control study. Iraqi J Med Sci. https://doi.org/10.22578/IJMS.21.2.8
doi: 10.22578/IJMS.21.2.8
Turin CG, Ochoa TJ (2014) The role of maternal breast milk in preventing infantile diarrhea in the developing world. Curr Tropic Med Rep 1:97–105. https://doi.org/10.1007/s40475-014-0015-x
doi: 10.1007/s40475-014-0015-x
Verma H, Chitambar SD, Gopalkrishna V (2011) Circulation of Aichi virus genotype B strains in children with acute gastroenteritis in India. Epidemiol Infect 139(11):1687–1691. https://doi.org/10.1017/S0950268810002979
doi: 10.1017/S0950268810002979
pubmed: 21211104
Verma H, Chitambar SD, Varanasi G (2009) Identification and characterization of enteric adenoviruses in infants and children hospitalized for acute gastroenteritis. J Med Virol 81(1):60–64. https://doi.org/10.1002/jmv.21331
doi: 10.1002/jmv.21331
pubmed: 19031470
Campos GS, Silva Sampaio ML, Menezes ADL, Tigre DM, Moura Costa LF, Chinalia FA et al (2016) Human bocavirus in acute gastroenteritis in children in Brazil. J Med Virol 1:166–170. https://doi.org/10.1002/jmv.24293
doi: 10.1002/jmv.24293
Zhou T, Chen Y, Chen J, Hu P, Zheng T, Xu X et al (2017) Prevalence and clinical profile of human bocavirus in children with acute gastroenteritis in chengdu, west china, 2012–2013. J Med Virol 89(10):1743–1748. https://doi.org/10.1002/jmv.24787
doi: 10.1002/jmv.24787
pubmed: 28198551