LED therapy modulates M1/M2 macrophage phenotypes and mitigates dystrophic features in treadmill-trained mdx mice.
mdx mice
Calcium pathways
Dystrophic muscle
Exercise
Inflammatory process
Oxidative stress
Photobiomodation
Journal
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
ISSN: 1474-9092
Titre abrégé: Photochem Photobiol Sci
Pays: England
ID NLM: 101124451
Informations de publication
Date de publication:
04 Sep 2024
04 Sep 2024
Historique:
received:
03
05
2024
accepted:
21
08
2024
medline:
4
9
2024
pubmed:
4
9
2024
entrez:
3
9
2024
Statut:
aheadofprint
Résumé
The mdx mouse phenotype, aggravated by chronic exercise on a treadmill, makes this murine model more reliable for the study of Duchenne muscular dystrophy (DMD) and allows the efficacy of therapeutic interventions to be evaluated. This study aims to investigate the effects of photobiomodulation by light-emitting diode (LED) therapy on functional, biochemical and morphological parameters in treadmill-trained adult mdx animals. Mdx mice were trained for 30 min of treadmill running at a speed of 12 m/min, twice a week for 4 weeks. The LED therapy (850 nm) was applied twice a week to the quadriceps muscle throughout the treadmill running period. LED therapy improved behavioral activity (open field) and muscle function (grip strength and four limb hanging test). Functional benefits correlated with reduced muscle damage; a decrease in the inflammatory process; modulation of the regenerative muscular process and calcium signalling pathways; and a decrease in oxidative stress markers. The striking finding of this work is that LED therapy leads to a shift from the M1 to M2 macrophage phenotype in the treadmill-trained mdx mice, enhancing tissue repair and mitigating the dystrophic features. Our data also imply that the beneficial effects of LED therapy in the dystrophic muscle correlate with the interplay between calcium, oxidative stress and inflammation signalling pathways. Together, these results suggest that photobiomodulation could be a potential adjuvant therapy for dystrophinopathies.
Identifiants
pubmed: 39227554
doi: 10.1007/s43630-024-00626-2
pii: 10.1007/s43630-024-00626-2
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2020/09733-4
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 140845/2020-8
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 130448/2022-2
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 303471/2022-0
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : 001
Informations de copyright
© 2024. The Author(s), under exclusive licence to the European Photochemistry Association, European Society for Photobiology.
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