Fibre-specific mitochondrial protein abundance is linked to resting and post-training mitochondrial content in the muscle of men.
Humans
Male
Mitochondrial Proteins
/ metabolism
Adult
Exercise
/ physiology
Proteomics
/ methods
Muscle, Skeletal
/ metabolism
Mitochondria, Muscle
/ metabolism
Young Adult
Muscle Fibers, Skeletal
/ metabolism
Rest
/ physiology
Mitochondria
/ metabolism
Proteome
/ metabolism
Adaptation, Physiological
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
03 Sep 2024
03 Sep 2024
Historique:
received:
16
11
2023
accepted:
16
07
2024
medline:
4
9
2024
pubmed:
4
9
2024
entrez:
3
9
2024
Statut:
epublish
Résumé
Analyses of mitochondrial adaptations in human skeletal muscle have mostly used whole-muscle samples, where results may be confounded by the presence of a mixture of type I and II muscle fibres. Using our adapted mass spectrometry-based proteomics workflow, we provide insights into fibre-specific mitochondrial differences in the human skeletal muscle of men before and after training. Our findings challenge previous conclusions regarding the extent of fibre-type-specific remodelling of the mitochondrial proteome and suggest that most baseline differences in mitochondrial protein abundances between fibre types reported by us, and others, might be due to differences in total mitochondrial content or a consequence of adaptations to habitual physical activity (or inactivity). Most training-induced changes in different mitochondrial functional groups, in both fibre types, were no longer significant in our study when normalised to changes in markers of mitochondrial content.
Identifiants
pubmed: 39227581
doi: 10.1038/s41467-024-50632-2
pii: 10.1038/s41467-024-50632-2
doi:
Substances chimiques
Mitochondrial Proteins
0
Proteome
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7677Informations de copyright
© 2024. The Author(s).
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