Tviblindi algorithm identifies branching developmental trajectories of human B-cell development and describes abnormalities in RAG-1 and WAS patients.
B‐cell development
CD73
Mass cytometry
RAG‐1
Trajectory inference
WAS
Journal
European journal of immunology
ISSN: 1521-4141
Titre abrégé: Eur J Immunol
Pays: Germany
ID NLM: 1273201
Informations de publication
Date de publication:
05 Sep 2024
05 Sep 2024
Historique:
revised:
23
08
2024
received:
11
01
2024
accepted:
27
08
2024
medline:
5
9
2024
pubmed:
5
9
2024
entrez:
5
9
2024
Statut:
aheadofprint
Résumé
Detailed knowledge of human B-cell development is crucial for the proper interpretation of inborn errors of immunity and malignant diseases. It is of interest to understand the kinetics of protein expression changes during development, but also to properly interpret the major and possibly alternative developmental trajectories. We have investigated human samples from healthy individuals with the aim of describing all B-cell developmental trajectories. We validated a 30-parameter mass cytometry panel and demonstrated the utility of "vaevictis" visualization of B-cell developmental stages. We used the trajectory inference tool "tviblindi" to exhaustively describe all trajectories leading to all developmental ends discovered in the data. Focusing on Natural Effector B cells, we demonstrated the dynamics of expression of nuclear factors (PAX-5, TdT, Ki-67, Bcl-2), cytokine and chemokine receptors (CD127, CXCR4, CXCR5) in relation to the canonical B-cell developmental stage markers. We observed branching of the memory development, where follicular memory formation was marked by CD73 expression. Lastly, we performed an analysis of two example cases of abnormal B-cell development caused by mutations in RAG-1 and Wiskott-Aldrich syndrome gene in patients with primary immunodeficiency. In conclusion, we developed, validated, and presented a comprehensive set of tools for the investigation of B-cell development in the bone marrow compartment.
Identifiants
pubmed: 39235410
doi: 10.1002/eji.202451004
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2451004Subventions
Organisme : The Czech Science Foundation
ID : 23-05561S
Organisme : Czech Health Research Council
ID : 23-07-00170
Organisme : European Union - Next Generation EU (Czech Recovery Plan) - Project National Cancer Research Institute
ID : LX22NPO5102
Organisme : FWO (Fonds Wetenschappelijk Onderzoek
ID : 1S40421N
Informations de copyright
© 2024 The Author(s). European Journal of Immunology published by Wiley‐VCH GmbH.
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