Mild Blast Exposure Dysregulates Metabolic Pathways and Correlation Networking as Evident from LC-MS-Based Plasma Profiling.
Blast-induced neurotrauma (BINT)
LC–MS
Metabolomics
Multiomics
Pathway
Traumatic brain injury (TBI)
Journal
Molecular neurobiology
ISSN: 1559-1182
Titre abrégé: Mol Neurobiol
Pays: United States
ID NLM: 8900963
Informations de publication
Date de publication:
05 Sep 2024
05 Sep 2024
Historique:
received:
18
01
2024
accepted:
08
08
2024
medline:
5
9
2024
pubmed:
5
9
2024
entrez:
5
9
2024
Statut:
aheadofprint
Résumé
Blast-induced trauma is emerging as a serious threat due to its wide pathophysiology where not only the brain but also a spectrum of organs is being affected. In the present study, we aim to identify the plasma-based metabolic dysregulations along with the associated temporal changes at 5-6 h, day 1 and day 7 post-injury in a preclinical animal model for blast exposure, through liquid chromatography-mass spectrometry (LC-MS). Using significantly advanced metabolomic and statistical bioinformatic platforms, we were able to elucidate better and unravel the complex networks of blast-induced neurotrauma (BINT) and its interlinked systemic effects. Significant changes were evident at 5-6 h with maximal changes at day 1. Temporal analysis also depicted progressive changes which continued till day 7. Significant associations of metabolic markers belonging to the class of amino acids, energy-related molecules, lipids, vitamin, hormone, phenolic acid, keto and histidine derivatives, nucleic acid molecules, uremic toxins, and uronic acids were observed. Also, the present study is the first of its kind where comprehensive, detailed pathway dysregulations of amino acid metabolism and biosynthesis, perturbed nucleotides, lipid peroxidation, and nucleic acid damage followed by correlation networking and multiomics networking were explored on preclinical animal models exposed to mild blast trauma. In addition, markers for systemic changes (renal dysfunction) were also observed. Global pathway predictions of unannotated peaks also presented important insights into BINT pathophysiology. Conclusively, the present study depicts important findings that might help underpin the biological mechanisms of blast-induced brain or systemic trauma.
Identifiants
pubmed: 39235645
doi: 10.1007/s12035-024-04429-5
pii: 10.1007/s12035-024-04429-5
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Department of Health Research, India
ID : (DHR)-YSF/DHR/12014/54/2020
Organisme : University Grants Commission
ID : UGC Grant
Organisme : University Grants Commission
ID : UGC Grant
Organisme : Council of Scientific and Industrial Research, India
ID : CSIR-Grant
Organisme : Defence R&D Organization (DRDO), Ministry of Defence, India
ID : INM3 24
Organisme : Defence R&D Organization (DRDO), Ministry of Defence, India
ID : INM3 24
Organisme : Defence R&D Organization (DRDO), Ministry of Defence, India
ID : INM3 24
Organisme : Defence R&D Organization (DRDO), Ministry of Defence, India
ID : INM3 24
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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