Monosome Stalls the Translation Process Mediated by IGF2BP in Arcuate Nucleus for Puberty Onset Delay.

ARC Hypothalamus IGF2BP2 Monosome Puberty Ribosome Translation regulation

Journal

Molecular neurobiology
ISSN: 1559-1182
Titre abrégé: Mol Neurobiol
Pays: United States
ID NLM: 8900963

Informations de publication

Date de publication:
05 Sep 2024
Historique:
received: 30 10 2023
accepted: 09 08 2024
medline: 5 9 2024
pubmed: 5 9 2024
entrez: 5 9 2024
Statut: aheadofprint

Résumé

Puberty onset through hypothalamic-pituitary-gonad (HPG) axis as an important reproductive event in postnatal development is initiated from hypothalamic arcuate nucleus (ARC). The growing evidence indicates that translational control also plays an essential role in the final expression of gonadotropin genes. To investigate the role of protein translation and behavior of ribosomes in pubertal onset, the global profiles of transcriptome, single ribosome (monosome), polysome, and tandem mass tag proteome were comprehensively investigated in rat hypothalamic ARCs of different pubertal stages using RNA sequencing, polyribo sequencing, and mass spectrum. Transcriptome-wide enrichments of N6-methyladenosine and IGF2BP2 were investigated using meRIP and RIP sequencing. Monosome was robustly enriched on a large proportion of mRNA in early puberty rats (postnatal day (PND)-25) compared to late puberty (PND-35 and PND-45). Monosome-enriched mRNAs, including HPG axis-related genes, had a large number of upstream ORFs (uORF, < 100 nt) and displayed translational repression in early puberty. Furthermore, insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) could particularly interact with and facilitate monosome to bind with mRNA in early puberty. Finally, ectopic over-expression of IGF2BP2 in hypothalamic ARC via lateral ventricle injection in vivo could recruit monosome to aggregate on mRNA and delay puberty onset. We uncovered a novel regulatory mechanism of IGF2BP2 and monosome for translational control in puberty onset, which shed light on the neuroendocrine regulatory network involved in HPG axis activation.

Identifiants

DOI: 10.1007/s12035-024-04450-8 PMID: 39235646
pubmed: 39235646
doi: 10.1007/s12035-024-04450-8
pii: 10.1007/s12035-024-04450-8
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : National Natural Science Foundation of China
ID : 82201797
Organisme : Natural Science Foundation of Shanghai Municipality
ID : 22ZR1451900
Organisme : Jiangsu Province High-level Innovation and Entrepreneurship Talent Plan
ID : JSSCBS20222022
Organisme : Suzhou Science and Technology Planning Project
ID : SKYD2022023
Organisme : Suzhou Science and Technology Planning Project
ID : SKJY2021016
Organisme : Gusu Health Talent Training Project
ID : GSWS2019087
Organisme : Gusu Health Talent Training Project
ID : GSWS2022111
Organisme : Science and Technology Planning Fund of Affiliated Hospital of Xuzhou Medical University
ID : XYFY202219
Organisme : Science and Technology Planning Fund of Affiliated Hospital of Xuzhou Medical University
ID : XYFY202306
Organisme : Science and Technology Planning Fund of Affiliated Hospital of Xuzhou Medical University
ID : XYFM202217
Organisme : Program of Developing Public Health through Science and Education of Wujiang District, Suzhou
ID : wwk202306
Organisme : Russian Science Foundation
ID : 24-24-00563

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Yifen Shen (Y)

Central Laboratory, Suzhou Bay Clinical College, Xuzhou Medical University, Suzhou Ninth People's Hospital, Soochow University, Suzhou, 215200, Jiangsu, China.

Le Zhang (L)

Jiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology, Suzhou Vocational Health College, Suzhou, 215009, Jiangsu, China.
Bio-ID Center, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.

Tao Yang (T)

Department of Medical Cosmetology, Suzhou Ninth People's Hospital, Soochow University, Suzhou, 215200, Jiangsu, China.

Xiaosong Li (X)

Department of Anorectal Surgery, Suzhou Ninth People's Hospital, Suzhou, 215200, Jiangsu, China.

Chao Liu (C)

Central Laboratory, Suzhou Bay Clinical College, Xuzhou Medical University, Suzhou Ninth People's Hospital, Soochow University, Suzhou, 215200, Jiangsu, China.

Hongmei Li (H)

Shanghai Institute of Precision Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200023, China.

Yanping Hu (Y)

Department of Molecular Pathology, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, 450008, Henan, China.

Hao Shen (H)

Clinical Laboratory, Suzhou Ninth People's Hospital, Suzhou, 215200, Jiangsu, China.

Hua Li (H)

Jiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology, Suzhou Vocational Health College, Suzhou, 215009, Jiangsu, China. kaikaixinxin@sjtu.edu.cn.

Yuriy L Orlov (YL)

The Digital Health Center, Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Moscow, 119991, Russia. orlov@d-health.institute.
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia. orlov@d-health.institute.

Shasha Zhou (S)

Department of Endocrinology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, 200040, China. zhoushasha@sjtu.edu.cn.

Yihang Shen (Y)

Central Laboratory, Suzhou Bay Clinical College, Xuzhou Medical University, Suzhou Ninth People's Hospital, Soochow University, Suzhou, 215200, Jiangsu, China. devbrother@sjtu.edu.cn.
ORCID: 0000-0002-2923-1641

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