Characterization of the membrane vesicle fraction from Acetobacter sp. WSS15.

Acetobacter sp. WSS15 Interleukin-6 Kurozu Membrane vesicles Peptidoglycan-associated lipoprotein Toll-like receptor 2

Journal

Journal of bioscience and bioengineering
ISSN: 1347-4421
Titre abrégé: J Biosci Bioeng
Pays: Japan
ID NLM: 100888800

Informations de publication

Date de publication:
05 Sep 2024
Historique:
received: 02 03 2024
revised: 14 07 2024
accepted: 26 07 2024
medline: 7 9 2024
pubmed: 7 9 2024
entrez: 6 9 2024
Statut: aheadofprint

Résumé

A bacterium that produces membrane vesicles (MVs), strain WSS15, was isolated from a traditional vinegar in Japan called Kurozu. A phylogenetic analysis of 16S rRNA gene sequences indicated that this bacterium belongs to the genus Acetobacter. MVs and peptidoglycan-associated lipoprotein (Pal) were detected in the MV fraction of strain WSS15. In the presence of the WSS15 MV fraction, murine macrophages produced the pro-inflammatory cytokine interleukin-6 (IL-6) via the recognition by superficial Toll-like receptor 2 (TLR2). WSS15 MVs adhered to the cell surface of macrophages. The macrophages secreted IL-6 through the TLR2 recognition of an acylated N-terminal peptide of Pal. We elucidated the mode of action of WSS15 MVs on immune cells and identified the Pal peptide from strain WSS15 as an agonist of TLR2.

Identifiants

DOI: 10.1016/j.jbiosc.2024.07.017 PMID: 39242325
pubmed: 39242325
pii: S1389-1723(24)00228-7
doi: 10.1016/j.jbiosc.2024.07.017
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2024 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Auteurs

Atsushi Kurata (A)

Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan. Electronic address: kurata090401@nara.kindai.ac.jp.

Kota Aimatsu (K)

Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan.

Yuki Kimura (Y)

Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan.

Hinako Hashiguchi (H)

Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan.

Asami Maeda (A)

Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan.

Tomoya Imai (T)

Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan.

Shino Yamasaki-Yashiki (S)

Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.

Kensaku Hamada (K)

Sakamoto Kurozu, Inc., 21-15 Uenosono-cho, Kagoshima, Kagoshima 890-0052, Japan.

Yuki Fujimoto (Y)

Sakamoto Kurozu, Inc., 21-15 Uenosono-cho, Kagoshima, Kagoshima 890-0052, Japan.

Akira Fujii (A)

Sakamoto Kurozu, Inc., 21-15 Uenosono-cho, Kagoshima, Kagoshima 890-0052, Japan.

Koichi Uegaki (K)

Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan; Agricultural Technology and Innovation Research Institute, Kindai University, 204-3327 Nakamachi, Nara, Nara 631-8505, Japan.

Classifications MeSH