Athyrium yokoscense, a cadmium-hypertolerant fern, exhibits two cadmium stress mitigation strategies in its roots and aerial parts.
Cd-O and Cd-S compounds
Chemical forms
Hypertolerance to Cd
Organic acids
Thiol compounds
XAFS and SR-XRF analysis
Journal
Journal of plant research
ISSN: 1618-0860
Titre abrégé: J Plant Res
Pays: Japan
ID NLM: 9887853
Informations de publication
Date de publication:
06 Sep 2024
06 Sep 2024
Historique:
received:
16
12
2023
accepted:
19
08
2024
medline:
7
9
2024
pubmed:
7
9
2024
entrez:
6
9
2024
Statut:
aheadofprint
Résumé
Athyrium yokoscense is hypertolerant to cadmium (Cd) and can grow normally under a high Cd concentration despite Cd being a highly toxic heavy metal. To mitigate Cd stress in general plant species, Cd is promptly chelated with a thiol compound and is isolated into vacuoles. Generated active oxygen species (ROS) in the cytoplasm are removed by reduced glutathione. However, we found many differences in the countermeasures in A. yokoscense. Thiol compounds accumulated in the stele of the roots, although a long-term Cd exposure induced Cd accumulation in the aerial parts. Synchrotron radiation-based X-ray fluorescence (SR-XRF) analysis indicated that a large amount of Cd was localized in the cell walls of the roots. Overexpression of AyNramp5a, encoding a representative Fe and Mn transporter of A. yokoscense, increased both Cd uptake and Fe and Mn uptake in rice calli under the Cd exposure conditions. Organic acids are known to play a key role in reducing Cd availability to the plants by forming chelation and preventing its entry in free form into the roots. In A. yokoscense roots, Organic acids were abundantly detected. Investigating the chemical forms of the Cd molecules by X-ray absorption fine structure (XAFS) analysis detected many compounds with Cd-oxygen (Cd-O) binding in A. yokoscense roots, whereas in the aerial parts, the ratio of the compounds with Cd-sulfur (Cd-S) binding was increased. Together, our results imply that the strong Cd tolerance of A. yokoscense is an attribute of the following two mechanisms: Cd-O compound formation in the cell wall is a barrier to reduce Cd uptake into aerial parts. Thiol compounds in the region of root stele are involved in detoxication of Cd by formation of Cd-S compounds.
Identifiants
pubmed: 39242481
doi: 10.1007/s10265-024-01574-9
pii: 10.1007/s10265-024-01574-9
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Synchrotron Radiation Research Institute
ID : 2012A1498
Organisme : Japan Synchrotron Radiation Research Institute
ID : 2011A1432
Organisme : Japan Synchrotron Radiation Research Institute
ID : 2011A1457
Organisme : Japan Synchrotron Radiation Research Institute
ID : 2010A1673
Organisme : Photon Factory Program Advisory Committee
ID : 2020G608
Informations de copyright
© 2024. The Author(s) under exclusive licence to The Botanical Society of Japan.
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