Genetic polymorphisms in FABP2, CYP2E1, and TP53 genes are potentially associated with colorectal cancer susceptibility.

Humans Colorectal Neoplasms / genetics Polymorphism, Single Nucleotide Male Female Genetic Predisposition to Disease Cytochrome P-450 CYP2E1 / genetics Fatty Acid-Binding Proteins / genetics Middle Aged Tumor Suppressor Protein p53 / genetics Case-Control Studies Cross-Sectional Studies Aged Adult Pakistan / epidemiology Genotype
CYP2E1 gene Colorectal cancer FABP2 gene Genetic polymorphisms Pakistani population TP53 gene

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
03 Sep 2024
Historique:
received: 05 02 2024
accepted: 16 08 2024
medline: 7 9 2024
pubmed: 7 9 2024
entrez: 6 9 2024
Statut: epublish

Résumé

Colorectal cancer (CRC) is among the most prevalent cancers with a high mortality rate. Both genetic and environmental factors contribute to CRC development. This study aimed to assess the association of single nucleotide polymorphisms (SNPs) in the fatty acid binding protein-2 (rs1799883), Cytochrome P450 2E1 (rs3813865), TP53 (rs1042522), and Murine double minute 2 (rs1042522) genes with CRC. A cross-sectional case-control study was conducted at the Institute of Molecular Biology and Biotechnology from May 2020 to March 2021, involving CRC patients (N = 100) and controls (N = 100) recruited from the Multan district in Pakistan. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) were employed to investigate the studied SNPs. The association of SNPs in all genes with CRC was examined either individually or in various combinations. Genotypes at three SNPs, rs1799883 in FABP2, rs3813865 in CYP2E1, and rs1042522 in TP53, were found to be associated with the development of CRC, while rs1042522 in MDM2 was not. Patients who were married, smoked, lacked exercise habits or had a family history of CRC were at a greater risk of acquiring the disease. FABP2 gene rs1799883, CYP2E1 gene rs3813865, and TP53 gene rs1042522 polymorphisms are significant in the development of CRC in Pakistani participants.

Identifiants

DOI: 10.1038/s41598-024-70381-y PMID: 39242607
pubmed: 39242607
doi: 10.1038/s41598-024-70381-y
pii: 10.1038/s41598-024-70381-y
doi:

Substances chimiques

Cytochrome P-450 CYP2E1 EC 1.14.13.-
Fatty Acid-Binding Proteins 0
FABP2 protein, human 0
Tumor Suppressor Protein p53 0
TP53 protein, human 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

20464

Informations de copyright

© 2024. The Author(s).

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Auteurs

Maryam Ijaz (M)

Institute of Zoology, Bahauddin Zakariya University, Multan, 60800, Pakistan.

Chien-Chin Chen (CC)

Department of Pathology, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi, 60002, Taiwan.
Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, 71710, Taiwan.
Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, 701, Taiwan.
Ph.D. Program in Translational Medicine, Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, 40227, Taiwan.

Rana Khalid Iqbal (RK)

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, 60800, Pakistan.
Department of Biology, University of Padova, Padua, Italy.

Hafiza Aneela Farooq (HA)

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, 60800, Pakistan.

Rubaida Mehmood (R)

Minar Multan Institute of Nuclear Medicine and Radiology Hospital, Multan, 60800, Pakistan.

Muhammad Asif (M)

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, 60800, Pakistan.

Atif Akbar (A)

Department of Statistics, Bahauddin Zakariya University, Multan, 60800, Pakistan.

Adil Khan (A)

Department of Botany and Zoology, Bacha Khan University, Charsadda, 24420, Khyber Pakhtunkhwa, Pakistan.

Waseem Ijaz (W)

University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan.

Mourad Ben Said (MB)

Laboratory of Microbiology, National School of Veterinary Medicine of Sidi Thabet, University of Manouba, 2010, Manouba, Tunisia. bensaidmourad8@yahoo.fr.
Department of Basic Sciences, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, 2010, Manouba, Tunisia. bensaidmourad8@yahoo.fr.

Gezahign Fentahun Wondmie (GF)

Department of Biology, Bahir Dar University, P.O.Box 79, Bahir Dar, Ethiopia. resercherfent@gmail.com.

Samir Ibenmoussa (S)

Laboratory of Therapeutic and Organic Chemistry, Faculty of Pharmacy, University of Montpellier, 34000, Montpellier, France.

Mohammad K Okla (MK)

Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, 11451, Riyadh, Saudi Arabia.

Furhan Iqbal (F)

Institute of Zoology, Bahauddin Zakariya University, Multan, 60800, Pakistan. furhan.iqbal@bzu.edu.pk.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Genetic polymorphisms in FABP2, CYP2E1, and...
questionsmedicales.fr › Maladies de l'appareil digestif › Maladies gastro-intestinales › Maladies intestinales › Maladies du rectum › Tumeurs colorectales › Genetic polymorphisms in FABP2, CYP2E1, and...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Polymorphisme génétique › Polymorphisme de nucléotide simple › Genetic polymorphisms in FABP2, CYP2E1, and...
questionsmedicales.fr › Phénomènes génétiques › Génotype › Prédisposition génétique à une maladie › Genetic polymorphisms in FABP2, CYP2E1, and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Hémoprotéines › Cytochromes › Cytochrome P-450 enzyme system › Famille-2 de cytochromes P450 › Cytochrome P-450 CYP2E1 › Genetic polymorphisms in FABP2, CYP2E1, and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de transport › Protéines de liaison aux acides gras › Genetic polymorphisms in FABP2, CYP2E1, and...
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Adulte d'âge moyen › Genetic polymorphisms in FABP2, CYP2E1, and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Phosphoprotéines › Protéine p53 suppresseur de tumeur › Genetic polymorphisms in FABP2, CYP2E1, and...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Caractéristiques des études épidémiologiques › Études épidémiologiques › Études cas-témoins › Genetic polymorphisms in FABP2, CYP2E1, and...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Caractéristiques des études épidémiologiques › Études épidémiologiques › Études transversales › Genetic polymorphisms in FABP2, CYP2E1, and...
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Sujet âgé › Genetic polymorphisms in FABP2, CYP2E1, and...
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Genetic polymorphisms in FABP2, CYP2E1, and...
questionsmedicales.fr › Régions géographiques › Asie › Asie de l'Ouest › Asie du Sud › Pakistan › Genetic polymorphisms in FABP2, CYP2E1, and...
questionsmedicales.fr › Phénomènes génétiques › Génotype › Genetic polymorphisms in FABP2, CYP2E1, and...