Chrysin downregulates the expression of ADAMTS-4 in foam cells.
ADAMTS-4
Atherosclerosis
Chrysin
Foam cells
Oxidized LDL
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
09 Sep 2024
09 Sep 2024
Historique:
received:
28
06
2024
accepted:
27
08
2024
medline:
9
9
2024
pubmed:
9
9
2024
entrez:
9
9
2024
Statut:
epublish
Résumé
Chrysin, a polyphenolic compound, possesses antioxidant and anti-inflammatory properties. In this study, we investigated the effect of chrysin on the expression of A disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), a protease enzyme involved in degrading extracellular matrix associated with atherosclerosis. We have studied the cell viability by MTT assay and foam cell formation by oil red O staining. The mRNA and protein expression of ADAMTS-4 was studied using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Our study showed that chrysin significantly downregulates the expression of ADAMTS-4 in foam cells. Chrysin's ability to downregulate the expression of ADAMTS-4, a protease involved in degrading the extracellular matrix, bestows upon it a new therapeutic potential for managing atherosclerosis.
Sections du résumé
BACKGROUND
BACKGROUND
Chrysin, a polyphenolic compound, possesses antioxidant and anti-inflammatory properties. In this study, we investigated the effect of chrysin on the expression of A disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), a protease enzyme involved in degrading extracellular matrix associated with atherosclerosis.
METHODS AND RESULTS
RESULTS
We have studied the cell viability by MTT assay and foam cell formation by oil red O staining. The mRNA and protein expression of ADAMTS-4 was studied using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Our study showed that chrysin significantly downregulates the expression of ADAMTS-4 in foam cells.
CONCLUSION
CONCLUSIONS
Chrysin's ability to downregulate the expression of ADAMTS-4, a protease involved in degrading the extracellular matrix, bestows upon it a new therapeutic potential for managing atherosclerosis.
Identifiants
pubmed: 39249599
doi: 10.1007/s11033-024-09896-6
pii: 10.1007/s11033-024-09896-6
doi:
Substances chimiques
Flavonoids
0
ADAMTS4 Protein
EC 3.4.24.82
chrysin
3CN01F5ZJ5
ADAMTS4 protein, human
EC 3.4.24.82
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
968Subventions
Organisme : Joint Council of Scientific & Industrial Research- University Grant Commission
ID : 361524, 21/06/2015(i)F4-V
Organisme : Joint Council of Scientific & Industrial Research- University Grant Commission
ID : 371532, 21/12/2014(ii)EU-V
Organisme : the Joint Council of Scientific & Industrial Research- University Grant Commission
ID : KL1716200565, 20/07/2020
Organisme : Kerala State Council for Science, Technology and Environment
ID : 03/FSHP/2013/CSTE
Organisme : University Grants Commission- Basic Scientific Research
ID : F.20-27/2013
Organisme : Department of Science and Technology-Science Engineering Research Board
ID : SB/YS/LS-55/2014
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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