The effect of combinations of a glyphosate-based herbicide with various clinically used antibiotics on phenotypic traits of Gram-negative species from the ESKAPEE group.
Glyphosate
Glycine
/ analogs & derivatives
Anti-Bacterial Agents
/ pharmacology
Herbicides
/ pharmacology
Microbial Sensitivity Tests
Gram-Negative Bacteria
/ drug effects
Acinetobacter baumannii
/ drug effects
Klebsiella pneumoniae
/ drug effects
Humans
Escherichia coli
/ drug effects
Pseudomonas aeruginosa
/ drug effects
Drug Resistance, Multiple, Bacterial
/ drug effects
Ciprofloxacin
/ pharmacology
Enterococcus faecium
/ drug effects
Staphylococcus aureus
/ drug effects
Colistin
/ pharmacology
Vancomycin
/ pharmacology
Enterobacter
/ drug effects
Drug Synergism
Meropenem
/ pharmacology
Phenotype
Gentamicins
/ pharmacology
Antibiotics
Carbapenem
ESKAPEE group
Glyphosate-based herbicide
Gram-negative bacteria
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
09 09 2024
09 09 2024
Historique:
received:
28
11
2023
accepted:
30
07
2024
medline:
10
9
2024
pubmed:
10
9
2024
entrez:
9
9
2024
Statut:
epublish
Résumé
The emission of glyphosate and antibiotic residues from human activities threatens the diversity and functioning of the microbial community. This study examines the impact of a glyphosate-based herbicide (GBH) and common antibiotics on Gram-negative bacteria within the ESKAPEE group (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. and Escherichia coli). Ten strains, including type and multidrug-resistant strains for each species were analysed and eight antibiotics (cefotaxime, meropenem, aztreonam, ciprofloxacin, gentamicin, tigecycline, sulfamethoxazole-trimethoprim, and colistin) were combined with the GBH. While most combinations yielded additive or indifferent effects in 70 associations, antagonistic effects were observed with ciprofloxacin and gentamicin in five strains. GBH notably decreased the minimum inhibitory concentration of colistin in eight strains and displayed synergistic activity with meropenem against metallo-β-lactamase (MBL)-producing strains. Investigation into the effect of GBH properties on outer membrane permeability involved exposing strains to a combination of this GBH and vancomycin. Results indicated that GBH rendered strains sensitive to vancomycin, which is typically ineffective against Gram-negative bacteria. Furthermore, we examined the impact of GBH in combination with three carbapenem agents on 14 strains exhibiting varying carbapenem-resistance mechanisms to assess its effect on carbapenemase activity. The GBH efficiently inhibited MBL activity, demonstrating similar effects to EDTA (ethylenediaminetetraacetic acid). Chelating effect of GBH may have multifaceted impacts on bacterial cells, potentially by increasing outer membrane permeability and inactivating metalloenzyme activity.
Identifiants
pubmed: 39251613
doi: 10.1038/s41598-024-68968-6
pii: 10.1038/s41598-024-68968-6
doi:
Substances chimiques
Glyphosate
4632WW1X5A
Glycine
TE7660XO1C
Anti-Bacterial Agents
0
Herbicides
0
Ciprofloxacin
5E8K9I0O4U
Colistin
Z67X93HJG1
Vancomycin
6Q205EH1VU
Meropenem
FV9J3JU8B1
Gentamicins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
21006Informations de copyright
© 2024. The Author(s).
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