Seminal plasma inhibits Chlamydia trachomatis infection in vitro, and may have consequences on mucosal immunity.

Chlamydia trachomatis / immunology Humans Female Semen / immunology Chlamydia Infections / immunology Neutrophils / immunology Cytokines / metabolism Male Immunity, Mucosal Epithelial Cells / microbiology Phagocytosis Cervix Uteri / microbiology
C. trachomatis Female mucosa Inflammation Neutrophil STI Seminal plasma

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
09 09 2024
Historique:
received: 12 10 2023
accepted: 28 08 2024
medline: 10 9 2024
pubmed: 10 9 2024
entrez: 9 9 2024
Statut: epublish

Résumé

Seminal plasma (SP) is the main vector of C. trachomatis (CT) during heterosexual transmission from male to female. It has immunomodulatory properties and impacts the susceptibility to HIV-1 infection, but its role has not been explored during CT infection. In the female reproductive tract (FRT), CT infection induces cytokine production and neutrophil recruitment. The role of neutrophils during CT infection is partially described, they could be at the origin of the pathology observed during CT infection. During this study, we developed an experimental in vitro model to characterize the impact of CT infection and SP on endocervical epithelial cell immune response in the FRT. We also studied the impact of the epithelial cell response on neutrophil phenotype and functions. We showed that the production by epithelial cells of pro-inflammatory cytokines increased during CT infection. Moreover, the pool of SP as well as individuals SP inhibited CT infection in a dose-dependent manner. The pool of SP inhibited cytokine production in a dose-dependent manner. The pool of SP altered gene expression profiles of infected cells. The culture supernatants of cells infected or not with CT, in presence or not of the pool of SP, had an impact on neutrophil phenotype and functions: they affected markers of neutrophil maturation, activation and adhesion capacity, as well as the survival, ROS production and phagocytosis ability. This study proposes a novel approach to study the impact of the environment on the phenotype and functions of neutrophils in the FRT. It highlights the impact of the factors of the FRT environment, in particular SP and CT infection, on the mucosal inflammation and the need to take into account the SP component while studying sexually transmitted infections during heterosexual transmission from male to female.

Identifiants

DOI: 10.1038/s41598-024-71499-9 PMID: 39251689
pubmed: 39251689
doi: 10.1038/s41598-024-71499-9
pii: 10.1038/s41598-024-71499-9
doi:

Substances chimiques

Cytokines 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

21050

Subventions

Organisme : Agence Nationale de Recherches sur le Sida et les Hépatites Virales
ID : AO 2020-1

Informations de copyright

© 2024. The Author(s).

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Auteurs

Louis Reot (L)

Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases [IMVA-HB/Infectious Disease Models and Innovative Therapies (IDMIT)], Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Inserm, Université Paris-Saclay, Fontenay-aux-Roses, France.

Cindy Adapen (C)

Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases [IMVA-HB/Infectious Disease Models and Innovative Therapies (IDMIT)], Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Inserm, Université Paris-Saclay, Fontenay-aux-Roses, France.

Claude Cannou (C)

Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases [IMVA-HB/Infectious Disease Models and Innovative Therapies (IDMIT)], Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Inserm, Université Paris-Saclay, Fontenay-aux-Roses, France.
Mucosal Immunity and Sexually Transmitted Infection Control (MISTIC) Group, Department of Virology, Institut Pasteur, Université Paris Cité, Paris, France.

Natalia Nunez (N)

Life&Soft, Fontenay-aux-Roses, France.

Sabrine Lakoum (S)

Life&Soft, Fontenay-aux-Roses, France.

Camille Pimienta (C)

Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases [IMVA-HB/Infectious Disease Models and Innovative Therapies (IDMIT)], Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Inserm, Université Paris-Saclay, Fontenay-aux-Roses, France.

Laetitia Lacroix (L)

Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases [IMVA-HB/Infectious Disease Models and Innovative Therapies (IDMIT)], Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Inserm, Université Paris-Saclay, Fontenay-aux-Roses, France.

Olivier Binois (O)

Service de Biologie de la Reproduction CECOS, Assistance Publique Hôpitaux de Paris, Hôpital Antoine Béclère, Université Paris-Saclay, Clamart, France.

Nelly Frydman (N)

Service de Biologie de la Reproduction CECOS, Assistance Publique Hôpitaux de Paris, Hôpital Antoine Béclère, Université Paris-Saclay, Clamart, France.

Marie-Thérèse Nugeyre (MT)

Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases [IMVA-HB/Infectious Disease Models and Innovative Therapies (IDMIT)], Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Inserm, Université Paris-Saclay, Fontenay-aux-Roses, France.
Mucosal Immunity and Sexually Transmitted Infection Control (MISTIC) Group, Department of Virology, Institut Pasteur, Université Paris Cité, Paris, France.

Roger Le Grand (R)

Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases [IMVA-HB/Infectious Disease Models and Innovative Therapies (IDMIT)], Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Inserm, Université Paris-Saclay, Fontenay-aux-Roses, France.

Elisabeth Menu (E)

Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases [IMVA-HB/Infectious Disease Models and Innovative Therapies (IDMIT)], Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Inserm, Université Paris-Saclay, Fontenay-aux-Roses, France. elisabeth.menu@cea.fr.
Mucosal Immunity and Sexually Transmitted Infection Control (MISTIC) Group, Department of Virology, Institut Pasteur, Université Paris Cité, Paris, France. elisabeth.menu@cea.fr.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Chlamydiales › Chlamydiaceae › Chlamydia › Chlamydia trachomatis › Seminal plasma inhibits Chlamydia trachomatis...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Seminal plasma inhibits Chlamydia trachomatis...
questionsmedicales.fr › Liquides et sécrétions biologiques › Sécrétions corporelles › Sperme › Seminal plasma inhibits Chlamydia trachomatis...
questionsmedicales.fr › Maladies urogénitales › Maladies génitales › Infections à Chlamydia › Seminal plasma inhibits Chlamydia trachomatis...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Granulocytes neutrophiles › Seminal plasma inhibits Chlamydia trachomatis...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Seminal plasma inhibits Chlamydia trachomatis...
questionsmedicales.fr › Phénomènes du système immunitaire › Immunité › Immunité muqueuse › Seminal plasma inhibits Chlamydia trachomatis...
questionsmedicales.fr › Cellules › Cellules épithéliales › Seminal plasma inhibits Chlamydia trachomatis...
questionsmedicales.fr › Phénomènes du système immunitaire › Phagocytose › Seminal plasma inhibits Chlamydia trachomatis...
questionsmedicales.fr › Appareil urogénital › Système génital › Système génital de la femme › Utérus › Col de l'utérus › Seminal plasma inhibits Chlamydia trachomatis...