Standard operating procedure for the analysis of major ions in hydrothermal fluids by ion chromatography.

This standard operating procedure (SOP) describes an ion chromatography (IC) procedure for the major cations and anions in hydrothermal fluids. Hydrothermal fluids are aqueous solutions with a wide range of temperature, salinity, pH and ion species that can be used by microbial metabolism as electron donors and electron acceptors. Due to the high variability of the environmental physical-chemical parameters in these samples, we have developed this protocol taking into account the special features of the matrices analyzed. An Eco IC Metrohm system equipped with a conductivity detector was used. Calibration curves are linear in the 0.1 to 10 mg/L concentration range for cations Ca This manuscript details the standard operating procedure used for the determination of major cations and anions in hydrothermal fluids, which are characterized by a high variability of physico-chemical parameters. Due to the high variability of our samples, we customized the method to compensate for the variability on the physico-chemical parameters. The results are useful to interpret the geochemical processes and for understanding how these processes influence microbial diversity in geothermal systems.

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Copyright: © 2024 Correggia M et al.

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No competing interests were disclosed.