Phenotypic profiling of human induced regulatory T cells at early differentiation: insights into distinct immunosuppressive potential.
Humans
T-Lymphocytes, Regulatory
/ immunology
Cell Differentiation
Antigens, CD
/ metabolism
Integrin alpha Chains
/ metabolism
Forkhead Transcription Factors
/ metabolism
Phenotype
Hepatitis A Virus Cellular Receptor 2
/ metabolism
Immune Tolerance
Receptors, Immunologic
/ metabolism
Proteomics
/ methods
Receptors, CXCR3
/ metabolism
Lymphocyte Activation Gene 3 Protein
Cells, Cultured
CD103
Differentiation
FOXP3
Mass cytometry
Mass spectrometry
Regulatory T cells
Journal
Cellular and molecular life sciences : CMLS
ISSN: 1420-9071
Titre abrégé: Cell Mol Life Sci
Pays: Switzerland
ID NLM: 9705402
Informations de publication
Date de publication:
12 Sep 2024
12 Sep 2024
Historique:
received:
26
06
2024
accepted:
27
08
2024
revised:
20
08
2024
medline:
12
9
2024
pubmed:
12
9
2024
entrez:
12
9
2024
Statut:
epublish
Résumé
Regulatory T cells (Tregs) play a key role in suppressing systemic effector immune responses, thereby preventing autoimmune diseases but also potentially contributing to tumor progression. Thus, there is great interest in clinically manipulating Tregs, but the precise mechanisms governing in vitro-induced Treg (iTreg) differentiation are not yet fully understood. Here, we used multiparametric mass cytometry to phenotypically profile human iTregs during the early stages of in vitro differentiation at single-cell level. A panel of 25 metal-conjugated antibodies specific to markers associated with human Tregs was used to characterize these immunomodulatory cells. We found that iTregs highly express the transcription factor FOXP3, as well as characteristic Treg-associated surface markers (e.g. CD25, PD1, CD137, CCR4, CCR7, CXCR3, and CD103). Expression of co-inhibitory factors (e.g. TIM3, LAG3, and TIGIT) increased slightly at late stages of iTreg differentiation. Further, CD103 was upregulated on a subpopulation of iTregs with greater suppressive capacity than their CD103
Identifiants
pubmed: 39264416
doi: 10.1007/s00018-024-05429-3
pii: 10.1007/s00018-024-05429-3
doi:
Substances chimiques
Antigens, CD
0
Integrin alpha Chains
0
Forkhead Transcription Factors
0
FOXP3 protein, human
0
alpha E integrins
0
TIGIT protein, human
0
Hepatitis A Virus Cellular Receptor 2
0
HAVCR2 protein, human
0
Lag3 protein, human
0
Receptors, Immunologic
0
Receptors, CXCR3
0
Lymphocyte Activation Gene 3 Protein
0
CXCR3 protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
399Informations de copyright
© 2024. The Author(s).
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