Transcriptome changes of liver fluke Opisthorchis viverrini in diabetic hamsters.
Modifications du transcriptome de la douve du foie Opisthorchis viverrini chez les hamsters diabétiques.
Diabetes mellitus
Host-parasite interactions
Liver fluke pathogenesis
Opisthorchis viverrini
Transcriptomics
Journal
Parasite (Paris, France)
ISSN: 1776-1042
Titre abrégé: Parasite
Pays: France
ID NLM: 9437094
Informations de publication
Date de publication:
2024
2024
Historique:
received:
07
09
2023
accepted:
27
08
2024
medline:
13
9
2024
pubmed:
13
9
2024
entrez:
13
9
2024
Statut:
ppublish
Résumé
A recent study in hamsters showed that infection with the liver fluke Opisthorchis viverrini in diabetic hosts worsens the severity of hepatobiliary disease. However, the effects of diabetes on the worm's phenotype and gene expression pattern remain unknown. This study investigated the impact of diabetes on the global gene expression and development of O. viverrini in diabetic hamsters. Parasitological parameters were assessed, and mRNA sequencing with bioinformatic analysis was performed. The study revealed that worm establishment rates in diabetic hamsters were directly correlated with fasting plasma glucose levels. Interestingly, worms collected from diabetic hosts exhibited stunted growth and reduced egg production. Transcriptomic analysis revealed significant alterations in gene expression, with 4314 and 567 differentially expressed genes at 21- and 35-days post-infection, respectively. Gene ontology enrichment analysis highlighted changes in biological processes related to stress response, metabolism, and cellular organization. Notably, genes associated with parasite virulence, including granulin, tetraspanins, and thioredoxins, showed significant upregulation in diabetic hosts. These findings demonstrate the profound impact of host diabetic status on O. viverrini development and gene expression, providing insights into the complex interplay between host metabolism and parasite biology, including molecular adaptations of O. viverrini in hosts. This study contributes to our understanding of opisthorchiasis in the context of metabolic disorders and may inform future strategies for disease management in diabetic human populations. Modifications du transcriptome de la douve du foie Opisthorchis viverrini chez les hamsters diabétiques. Une étude récente sur les hamsters a montré que l’infection par la douve du foie Opisthorchis viverrini chez les hôtes diabétiques aggrave la gravité de la maladie hépatobiliaire. Cependant, les effets du diabète sur le phénotype et le profil d’expression génétique du ver restent inconnus. Cette étude a examiné l’impact du diabète sur l’expression génétique globale et le développement d’O. viverrini chez les hamsters diabétiques. Les paramètres parasitologiques ont été évalués et un séquençage de l’ARNm avec analyse bioinformatique a été effectué. L’étude a révélé que les taux d’établissement des vers chez les hamsters diabétiques étaient directement corrélés au taux de glucose plasmatique à jeun. Il est intéressant de noter que les vers récupérés auprès d’hôtes diabétiques présentaient une croissance retardée et une production d’œufs réduite. L’analyse transcriptomique a révélé des altérations significatives de l’expression génétique, avec 4 314 et 567 gènes exprimés de manière différentielle à 21 et 35 jours après l’infection, respectivement. L’analyse d’enrichissement de l’ontologie génétique a mis en évidence des changements dans les processus biologiques liés à la réponse au stress, au métabolisme et à l’organisation cellulaire. Notamment, les gènes associés à la virulence du parasite, en particulier la granuline, les tétraspanines et les thiorédoxines, ont montré une régulation positive significative chez les hôtes diabétiques. Ces résultats démontrent l’impact profond du statut diabétique de l’hôte sur le développement et l’expression génétique d’O. viverrini, offrant un aperçu de l’interaction complexe entre le métabolisme de l’hôte et la biologie du parasite, y compris les adaptations moléculaires d’O. viverrini chez les hôtes. Cette étude contribue à notre compréhension de l’opisthorchiase dans le contexte des troubles métaboliques et peut éclairer les futures stratégies de gestion de la maladie pour les populations humaines diabétiques.
Autres résumés
Type: Publisher
(fre)
Modifications du transcriptome de la douve du foie Opisthorchis viverrini chez les hamsters diabétiques.
Identifiants
pubmed: 39269256
doi: 10.1051/parasite/2024056
pii: parasite230129
doi:
Substances chimiques
Blood Glucose
0
Granulins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
54Subventions
Organisme : Khon Kaen University
ID : Young Researcher Development Project of Khon Kaen University Year 2022
Organisme : Office of the Permanent Secretary, Ministry of Higher Education, Science, Research and Innovation, Thailand
ID : RGNS 64-052
Informations de copyright
© A. Chaidee et al., published by EDP Sciences, 2024.
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