Biomechanical validation of a tibial critical-size defect model in minipigs.

Biomechanics Critical-size bone defect Cyclic testing Fracture Minipigs

Journal

Clinical biomechanics (Bristol, Avon)
ISSN: 1879-1271
Titre abrégé: Clin Biomech (Bristol, Avon)
Pays: England
ID NLM: 8611877

Informations de publication

Date de publication:
07 Sep 2024
Historique:
received: 23 03 2024
revised: 03 09 2024
accepted: 04 09 2024
medline: 15 9 2024
pubmed: 15 9 2024
entrez: 14 9 2024
Statut: aheadofprint

Résumé

Autologous cancellous bone grafting still represents the gold standard for the therapy of non-healing bone defects. However, donor site morbidity and the restricted availability of autologous bone grafts have initiated scientists to look for promising alternatives to heal even large defects. The present study aimed to evaluate the biomechanical potential and failure properties of a previously developed metaphyseal critical-size defect model of the proximal tibia in minipigs for future comparisons of bone substitute materials. Fresh-frozen minipig tibiae were divided into two groups, with half undergoing the creation of critical-size defects. Specimens were subjected to biomechanical fatigue tests and load-to-failure tests. CT scans post-test verified bone damage. Statistical analysis compared the properties of defected and intact specimens. In this model, it was demonstrated that under uniaxial cyclic compression within the loading axis, the intact tibiae specimens (8708 ± 202 N) provided a significant (p = 0.014) higher compressive force to failure than the tibiae with the defect (6566 ± 1653 N). Thus, the used minipig model is suitable for comparing bone substitute materials regarding their biomechanical forces and bone regeneration capacity.

Sections du résumé

BACKGROUND BACKGROUND
Autologous cancellous bone grafting still represents the gold standard for the therapy of non-healing bone defects. However, donor site morbidity and the restricted availability of autologous bone grafts have initiated scientists to look for promising alternatives to heal even large defects. The present study aimed to evaluate the biomechanical potential and failure properties of a previously developed metaphyseal critical-size defect model of the proximal tibia in minipigs for future comparisons of bone substitute materials.
METHODS METHODS
Fresh-frozen minipig tibiae were divided into two groups, with half undergoing the creation of critical-size defects. Specimens were subjected to biomechanical fatigue tests and load-to-failure tests. CT scans post-test verified bone damage. Statistical analysis compared the properties of defected and intact specimens.
FINDINGS RESULTS
In this model, it was demonstrated that under uniaxial cyclic compression within the loading axis, the intact tibiae specimens (8708 ± 202 N) provided a significant (p = 0.014) higher compressive force to failure than the tibiae with the defect (6566 ± 1653 N).
INTERPRETATION CONCLUSIONS
Thus, the used minipig model is suitable for comparing bone substitute materials regarding their biomechanical forces and bone regeneration capacity.

Identifiants

pubmed: 39276502
pii: S0268-0033(24)00168-2
doi: 10.1016/j.clinbiomech.2024.106336
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

106336

Informations de copyright

Copyright © 2024. Published by Elsevier Ltd.

Déclaration de conflit d'intérêts

Declaration of competing interest All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers' bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.

Auteurs

Marx Ribeiro (M)

Department of Orthopedics, Trauma and Reconstructive Surgery University Hospital RWTH Aachen, Pauwelstr. 30, 52074 Aachen, Germany; Department of Trauma and Reconstructive Surgery University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), Germany. Electronic address: marx.ribeiro@rwth-aachen.de.

Vera Cora Grotheer (VC)

Department of Orthopedics and Trauma Surgery, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University, Moorenstr. 5, 40225 Düsseldorf, Germany. Electronic address: vera.grotheer@med.uni-duesseldorf.de.

Luis Fernando Nicolini (LF)

Department of Mechanical Engineering, Federal University of Santa Maria UFSM, Av. Roraima n° 1000 Cidade Universitária Bairro - Camobi, 97105 - 900 Santa Maria, Brazil. Electronic address: nicolini.luis@ufsm.br.

David Latz (D)

Department of Orthopedics and Trauma Surgery, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University, Moorenstr. 5, 40225 Düsseldorf, Germany. Electronic address: David.Latz@med.uni-duesseldorf.de.

Miguel Pishnamaz (M)

Department of Orthopedics, Trauma and Reconstructive Surgery University Hospital RWTH Aachen, Pauwelstr. 30, 52074 Aachen, Germany. Electronic address: mpishnamaz@ukaachen.de.

Johannes Greven (J)

Department of Thorax Surgery, University Hospital RWTH Aachen, Pauwelstr. 30, 52074 Aachen, Germany. Electronic address: jgreven@ukaachen.de.

Roman Taday (R)

Department of Orthopedics and Trauma Surgery, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University, Moorenstr. 5, 40225 Düsseldorf, Germany. Electronic address: Roman.Taday@med.uni-duesseldorf.de.

Niklas Markus Wergen (NM)

Department of Orthopedics and Trauma Surgery, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University, Moorenstr. 5, 40225 Düsseldorf, Germany. Electronic address: niklasmarkus.wergen@med.uni-duesseldorf.de.

Frank Hildebrand (F)

Department of Orthopedics, Trauma and Reconstructive Surgery University Hospital RWTH Aachen, Pauwelstr. 30, 52074 Aachen, Germany. Electronic address: fhildebrand@ukaachen.de.

Joachim Windolf (J)

Department of Orthopedics and Trauma Surgery, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University, Moorenstr. 5, 40225 Düsseldorf, Germany. Electronic address: Joachim.Windolf@med.uni-duesseldorf.de.

Pascal Jungbluth (P)

Department of Orthopedics and Trauma Surgery, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University, Moorenstr. 5, 40225 Düsseldorf, Germany. Electronic address: Pascal.Jungbluth@med.uni-duesseldorf.de.

Classifications MeSH