Nucleosomal DNA unwinding pathway through canonical and non-canonical histone disassembly.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
14 Sep 2024
14 Sep 2024
Historique:
received:
24
05
2024
accepted:
05
09
2024
medline:
15
9
2024
pubmed:
15
9
2024
entrez:
14
9
2024
Statut:
epublish
Résumé
The nucleosome including H2A.B, a mammalian-specific H2A variant, plays pivotal roles in spermatogenesis, embryogenesis, and oncogenesis, indicating unique involvement in transcriptional regulation distinct from canonical H2A nucleosomes. Despite its significance, the exact regulatory mechanism remains elusive. This study utilized solid-state nanopores to investigate DNA unwinding dynamics, applying local force between DNA and histones. Comparative analysis of canonical H2A and H2A.B nucleosomes demonstrated that the H2A.B variant required a lower voltage for complete DNA unwinding. Furthermore, synchronization analysis and molecular dynamics simulations indicate that the H2A.B variant rapidly unwinds DNA, causing the H2A-H2B dimer to dissociate from DNA immediately upon disassembly of the histone octamer. In contrast, canonical H2A nucleosomes unwind DNA at a slower rate, suggesting that the H2A-H2B dimer undergoes a state of stacking at the pore. These findings suggest that nucleosomal DNA in the H2A.B nucleosomes undergoes a DNA unwinding process involving histone octamer disassembly distinct from that of canonical H2A nucleosomes, enabling smoother unwinding. The integrated approach of MD simulations and nanopore measurements is expected to evolve into a versatile tool for studying molecular interactions, not only within nucleosomes but also through the forced dissociation of DNA-protein complexes.
Identifiants
pubmed: 39277674
doi: 10.1038/s42003-024-06856-5
pii: 10.1038/s42003-024-06856-5
doi:
Substances chimiques
Nucleosomes
0
Histones
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1144Subventions
Organisme : MEXT | Japan Science and Technology Agency (JST)
ID : JPMJCR2231
Organisme : MEXT | Japan Science and Technology Agency (JST)
ID : JPMJER2002
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP23H05475
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP24H02328
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 20J00261
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP24ama121009
Informations de copyright
© 2024. The Author(s).
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