Methods for the Inactivation of Mycobacterium tuberculosis: a Systematic Review of the Literature.


Journal

International journal of mycobacteriology
ISSN: 2212-554X
Titre abrégé: Int J Mycobacteriol
Pays: India
ID NLM: 101615660

Informations de publication

Date de publication:
01 Jul 2024
Historique:
received: 23 03 2024
accepted: 25 06 2024
medline: 15 9 2024
pubmed: 15 9 2024
entrez: 15 9 2024
Statut: ppublish

Résumé

To systematize published laboratory methods to inactivate Mycobacterium tuberculosis (MTB) and to describe their effectiveness. We carried out a review of the scientific literature to identify the publications that reported methods for the inactivation of MTB, according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses recommendations. The search addressed inactivation methodologies used in Public health laboratories for the treatment of biological material and only included studies reporting the efficacy of the method. The database used were PubMed (National Library of Medicine) and LILACS (Latin American and Caribbean Literature in Health Sciences). We evaluated the quality of the studies with the JBI (Joanna Briggs Institute) Critical Instrument Appraisal Checklist. We included 14 publications meeting the established inclusion and exclusion criteria. These 14 studies actually described a total of 35 inactivation methods. Most of them combined heat treatment with some chemical or enzymatic agent, and there were very few applying a single strategy. Twenty-four (68.57%) methods demonstrated significant efficacy in inactivating MTB. The systematic review identified 35 methods of inactivation of MTB, published in 14 studies. Most of the methods combined physical treatment techniques, especially heat, with chemical and/or enzymatic treatment techniques, obtaining mostly good results in preventing the reproduction of the microorganism. PROSPERO (International Prospective Register of Ongoing Systematic Reviews) (Code CRD42024503621).

Identifiants

pubmed: 39277884
doi: 10.4103/ijmy.ijmy_49_24
pii: 01929415-202413030-00002
doi:

Types de publication

Journal Article Systematic Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

237-246

Informations de copyright

Copyright © 2024 Copyright: © 2024 International Journal of Mycobacteriology.

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Auteurs

Aiko N Vigo (AN)

Laboratorio de Referencia Nacional de Micobacterias, Instituto Nacional de Salud, Lima, Perú.

Zully M Puyén (ZM)

Laboratorio de Referencia Nacional de Micobacterias, Instituto Nacional de Salud, Lima, Perú.

David Santos-Lázaro (D)

Laboratorio de Referencia Nacional de Micobacterias, Instituto Nacional de Salud, Lima, Perú.

Mary L Perea (ML)

Laboratorio Referencial Regional, Gerencia Regional de Salud Arequipa, Arequipa, Perú.

Lely Solari (L)

Laboratorio de Referencia Nacional de Micobacterias, Instituto Nacional de Salud, Lima, Perú.

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