RNA fine-tunes estrogen receptor-alpha binding on low-affinity DNA motifs for transcriptional regulation.
Chromatin
DNA-motifs
Estrogen Receptor-Alpha
Non-Coding RNA
Transcription Factors
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
16 Sep 2024
16 Sep 2024
Historique:
received:
29
08
2023
accepted:
15
07
2024
revised:
12
06
2024
medline:
17
9
2024
pubmed:
17
9
2024
entrez:
16
9
2024
Statut:
aheadofprint
Résumé
Transcription factors (TFs) regulate gene expression by binding with varying strengths to DNA via their DNA-binding domain. Additionally, some TFs also interact with RNA, which modulates transcription factor binding to chromatin. However, whether RNA-mediated TF binding results in differential transcriptional outcomes remains unknown. In this study, we demonstrate that estrogen receptor α (ERα), a ligand-activated TF, interacts with RNA in a ligand-dependent manner. Defects in RNA binding lead to genome-wide loss of ERα recruitment, particularly at weaker ERα-motifs. Furthermore, ERα mobility in the nucleus increases in the absence of its RNA-binding capacity. Unexpectedly, this increased mobility coincides with robust polymerase loading and transcription of ERα-regulated genes that harbor low-strength motifs. However, highly stable binding of ERα on chromatin negatively impacts ligand-dependent transcription. Collectively, our results suggest that RNA interactions spatially confine ERα on low-affinity sites to fine-tune gene transcription.
Identifiants
pubmed: 39284910
doi: 10.1038/s44318-024-00225-y
pii: 10.1038/s44318-024-00225-y
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Wellcome Trust DBT India Alliance (India Alliance)
ID : IA/S/23/1/506749
Organisme : Department of Science and Technology, Ministry of Science and Technology, India (DST)
ID : CRG/2019/005714
Organisme : European Molecular Biology Organization (EMBO)
ID : GIN
Organisme : TIFR | National Centre for Biological Sciences (NCBS)
ID : RTI 4006
Informations de copyright
© 2024. The Author(s).
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