The first detection of SARS-CoV-2 RNA in the wastewater of Bucharest, Romania.
Digital PCR
Next-generation sequencing
Phylogeny
SARS-CoV-2
Wastewater
Wastewater-based epidemiology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 Sep 2024
17 Sep 2024
Historique:
received:
01
05
2024
accepted:
11
09
2024
medline:
18
9
2024
pubmed:
18
9
2024
entrez:
17
9
2024
Statut:
epublish
Résumé
Wastewater-based epidemiology (WBE) has been previously used as a tool for pathogen identification within communities. After the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) outbreak, in 2020, Daughton proposed the implementation of a wastewater surveillance strategy that could determine the incidence of COVID-19 (coronavirus disease 2019) nationally. Individuals in various stages of SARS-CoV-2 infection, including presymptomatic, asymptomatic and symptomatic patients, can be identified as carriers of the virus in their urine, saliva, stool and other bodily secretions. Studies using this method were conducted to monitor the prevalence of the virus in high-density populations, such as cities but also in smaller communities, such as schools and college campuses. The aim of this pilot study was to assess the feasibility and effectiveness of wastewater surveillance in Bucharest, Romania, and wastewater samples were collected weekly from seven locations between July and September 2023. RNA (ribonucleic acid) extraction, followed by dPCR (digital polymerase chain reaction) analysis, was performed to detect viral genetic material. Additionally, NGS (next generation sequencing) technology was used to identify the circulating variants within the wastewater of Bucharest, Romania. Preliminary results indicate the successful detection of SARS-CoV-2 RNA in wastewater, providing valuable insights into the circulation of the virus within the community.
Identifiants
pubmed: 39289536
doi: 10.1038/s41598-024-72854-6
pii: 10.1038/s41598-024-72854-6
doi:
Substances chimiques
Wastewater
0
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
21730Subventions
Organisme : European Commission
ID : [060701/2021/864662/SUB/ENV]. C2]
Organisme : European Commission
ID : [060701/2021/864662/SUB/ENV]. C2]
Organisme : European Commission
ID : [060701/2021/864662/SUB/ENV]. C2]
Organisme : European Commission
ID : [060701/2021/864662/SUB/ENV]. C2]
Organisme : European Commission
ID : [060701/2021/864662/SUB/ENV]. C2]
Informations de copyright
© 2024. The Author(s).
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