High-throughput peptide array analysis and computational techniques for serological profiling of flavivirus infections: Implications for diagnostics and vaccine development.
Humans
Flavivirus Infections
/ diagnosis
Flavivirus
/ immunology
Protein Array Analysis
/ methods
Peptides
/ immunology
Vaccine Development
Computational Biology
/ methods
Dengue
/ diagnosis
Dengue Virus
/ immunology
High-Throughput Screening Assays
/ methods
Serologic Tests
/ methods
Biomarkers
/ blood
Viral Proteins
/ immunology
Adult
Antibodies, Viral
/ blood
Middle Aged
Male
Female
Zika Virus
/ immunology
dengue virus
epitope prediction
flavivirus
peptides
protein arrays
serodiagnosis
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
Sep 2024
Sep 2024
Historique:
revised:
28
08
2024
received:
05
04
2024
accepted:
05
09
2024
medline:
18
9
2024
pubmed:
18
9
2024
entrez:
18
9
2024
Statut:
ppublish
Résumé
Arthropod-borne viruses, such as dengue virus (DENV), pose significant global health threats, with DENV alone infecting around 400 million people annually and causing outbreaks beyond endemic regions. This study aimed to enhance serological diagnosis and discover new drugs by identifying immunogenic protein regions of DENV. Utilizing a comprehensive approach, the study focused on peptides capable of distinguishing DENV from other flavivirus infections through serological analyses. Over 200 patients with confirmed arbovirus infection were profiled using high-density pan flavivirus peptide arrays comprising 6253 peptides and the computational method matrix of local coupling energy (MLCE). Twenty-four peptides from nonstructural and structural viral proteins were identified as specifically recognized by individuals with DENV infection. Six peptides were confirmed to distinguish DENV from Zika virus (ZIKV), West Nile virus (WNV), Yellow Fever virus (YFV), Usutu virus (USUV), and Chikungunya virus (CHIKV) infections, as well as healthy controls. Moreover, the combination of two immunogenic peptides emerged as a potential serum biomarker for DENV infection. These peptides, mapping to highly accessible regions on protein structures, show promise for diagnostic and prophylactic strategies against flavivirus infections. The described methodology holds broader applicability in the serodiagnosis of infectious diseases.
Substances chimiques
Peptides
0
Biomarkers
0
Viral Proteins
0
Antibodies, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e29923Subventions
Organisme : Ministero della Salute
Organisme : European Commission
Organisme : Fondazione Romeo ed Enrica Invernizzi
Organisme : Regione Lombardia
Informations de copyright
© 2024 Wiley Periodicals LLC.
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