Bud14 function is crucial for spindle pole body size maintenance.

Background/aim

Spindle pole bodies (SPB), the functional equivalent of centrosomes in yeast, duplicate through generation of a new SPB next to the old one. However, SPBs are dynamic structures that can grow and exchange, and mechanisms that regulate SPB size remain largely unknown. This study aims to elucidate the role of Bud14 in SPB size maintenance in

Materials and methods

We employed quantitative fluorescence microscopy to assess the relative and absolute amounts of SPB structural proteins at SPBs of wildtype cells and in cells lacking

Results

Our results indicate that higher levels of SPB inner, outer, and central plaque proteins are present at the SPBs of

Conclusion

We identified Bud14 as a critical protein for SPB size maintenance. The interaction of Bud14 with Glc7, but not with the Kelch proteins, is indispensable for restricting levels of Spc110 incorporated into the SPBs.

Informations de copyright

© TÜBİTAK.

Déclaration de conflit d'intérêts

Conflict of interest: The authors declare that they have no conflicts of interest.