Non-thermal atmospheric pressure gas discharge plasma enhances tendon-to-bone junction repair in a rabbit model.

infraspinatus tendon irradiation non-thermal atmospheric pressure gas discharge plasma rotator cuff tear shoulder injury tendon-bone junction

Journal

Journal of shoulder and elbow surgery
ISSN: 1532-6500
Titre abrégé: J Shoulder Elbow Surg
Pays: United States
ID NLM: 9206499

Informations de publication

Date de publication:
17 Sep 2024
Historique:
received: 04 03 2024
revised: 16 07 2024
accepted: 25 07 2024
medline: 20 9 2024
pubmed: 20 9 2024
entrez: 19 9 2024
Statut: aheadofprint

Résumé

Plasma is a collection of active particles generated by dissociating molecules and ionizing atoms through applying high energy to a gas, such as high-sound heating or electrical shock. Recently, many reports have been published on the effectiveness of non-thermal atmospheric pressure gas discharge plasma (NTAPP) on living organisms. Furthermore, we have reported on the promotion of bone and tendon repair by NTAPP irradiation. We hypothesized that irradiation of NTAPP would promote the repair of the tendon-bone junction in a rotator cuff repair. This study investigated the effect of NTAPP irradiation on the healing process of the tendon-bone junction. Among 36 Japanese white rabbits, the infraspinatus tendon was detached from the humeral insertion site. A 3.2 mm bone tunnel was then created at the original insertion site of the infraspinatus muscle. The left shoulder was irradiated with NTAPP at a distance of 1 cm from the bone tunnel for 5 minutes (plasma-treated group), while the right shoulder was not irradiated (control group). The rabbits were sacrificed at 2, 4, and 8 weeks postoperatively, and six of each were used for histological evaluation. Mechanical tests were also performed on six specimens each at 4 and 8 weeks. Histological evaluation showed that at 4 weeks, the histological tendon to bone maturing score was 6.8±1.3 in the plasma-treated group and 4.8±1.6 in the control group (p<0.01); at 8 weeks it was 9.0±1.0 in the plasma-treated group and 5.2±1.1 in the control group (p<0.01). Fibrocartilage formation and new bone formation were observed at both 4 and 8 weeks. In the mechanical test, the plasma-treated group had 75.0 ± 18.9 N in ultimate load to failure at 8 weeks. In the control group, it was 51.1±7.9 N. (p=0.04) CONCLUSION: The repair of the rotator cuff at the tendon-bone junction was significantly improved at 4 and 8 weeks by irradiation with NTAPP.

Sections du résumé

BACKGROUND BACKGROUND
Plasma is a collection of active particles generated by dissociating molecules and ionizing atoms through applying high energy to a gas, such as high-sound heating or electrical shock. Recently, many reports have been published on the effectiveness of non-thermal atmospheric pressure gas discharge plasma (NTAPP) on living organisms. Furthermore, we have reported on the promotion of bone and tendon repair by NTAPP irradiation. We hypothesized that irradiation of NTAPP would promote the repair of the tendon-bone junction in a rotator cuff repair. This study investigated the effect of NTAPP irradiation on the healing process of the tendon-bone junction.
METHODS METHODS
Among 36 Japanese white rabbits, the infraspinatus tendon was detached from the humeral insertion site. A 3.2 mm bone tunnel was then created at the original insertion site of the infraspinatus muscle. The left shoulder was irradiated with NTAPP at a distance of 1 cm from the bone tunnel for 5 minutes (plasma-treated group), while the right shoulder was not irradiated (control group). The rabbits were sacrificed at 2, 4, and 8 weeks postoperatively, and six of each were used for histological evaluation. Mechanical tests were also performed on six specimens each at 4 and 8 weeks.
RESULTS RESULTS
Histological evaluation showed that at 4 weeks, the histological tendon to bone maturing score was 6.8±1.3 in the plasma-treated group and 4.8±1.6 in the control group (p<0.01); at 8 weeks it was 9.0±1.0 in the plasma-treated group and 5.2±1.1 in the control group (p<0.01). Fibrocartilage formation and new bone formation were observed at both 4 and 8 weeks. In the mechanical test, the plasma-treated group had 75.0 ± 18.9 N in ultimate load to failure at 8 weeks. In the control group, it was 51.1±7.9 N. (p=0.04) CONCLUSION: The repair of the rotator cuff at the tendon-bone junction was significantly improved at 4 and 8 weeks by irradiation with NTAPP.

Identifiants

DOI: 10.1016/j.jse.2024.07.039 PMID: 39299643
pubmed: 39299643
pii: S1058-2746(24)00640-2
doi: 10.1016/j.jse.2024.07.039
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2024. Published by Elsevier Inc.

Auteurs

Katsumasa Nakazawa (K)

Department of Orthopedic Surgery, Graduate School of Medicine, Osaka City University, Abeno, Osaka 545-8585, Japan.

Hiromitsu Toyoda (H)

Department of Orthopedic Surgery, Graduate School of Medicine, Osaka City University, Abeno, Osaka 545-8585, Japan; Department of Orthopedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Abeno, Osaka 545-8585, Japan. Electronic address: h-toyoda@omu.ac.jp.

Tomoya Manaka (T)

Department of Orthopedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Abeno, Osaka 545-8585, Japan.

Kumi Orita (K)

Department of Orthopedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Abeno, Osaka 545-8585, Japan.

Yoshihiro Hirakawa (Y)

Ishikiriseiki Hospital, Higashiosaka, Osaka 579-8026, Japan.

Yoichi Ito (Y)

Ito Clinic, Osaka Shoulder Center, Matsubara, Osaka 530-0012, Japan.

Kosuke Saito (K)

Department of Orthopedic Surgery, Graduate School of Medicine, Osaka City University, Abeno, Osaka 545-8585, Japan.

Ryosuke Iio (R)

Department of Orthopedic Surgery, Graduate School of Medicine, Osaka City University, Abeno, Osaka 545-8585, Japan.

Yoshitaka Ban (Y)

Department of Orthopedic Surgery, Graduate School of Medicine, Osaka City University, Abeno, Osaka 545-8585, Japan.

Hana Yao (H)

Department of Orthopedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Abeno, Osaka 545-8585, Japan.

Yuto Kobayashi (Y)

Department of Orthopedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Abeno, Osaka 545-8585, Japan.

Jun-Seok Oh (JS)

Department of Physics and Electronics, Graduate School of Engineering, Osaka Metropolitan University, Sumiyoshi, Osaka 558-8585, Japan.

Tatsuru Shirafuji (T)

Department of Physics and Electronics, Graduate School of Engineering, Osaka Metropolitan University, Sumiyoshi, Osaka 558-8585, Japan.

Hiroaki Nakamura (H)

Department of Orthopedic Surgery, Graduate School of Medicine, Osaka City University, Abeno, Osaka 545-8585, Japan; Department of Orthopedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Abeno, Osaka 545-8585, Japan.

Classifications MeSH