The selective proapoptotic impact of the myricetin-loaded alginate-cellulose hybrid nanocrystals (MAC-NCs) on the human AGS gastric cancer cells.
Humans
Alginates
/ chemistry
Stomach Neoplasms
/ drug therapy
Apoptosis
/ drug effects
Nanoparticles
/ chemistry
Cell Line, Tumor
Cellulose
/ pharmacology
Flavonoids
/ pharmacology
Caspase 9
/ metabolism
Caspase 8
/ metabolism
Human Umbilical Vein Endothelial Cells
/ drug effects
Cell Survival
/ drug effects
Anti-gastric cancer
Apoptosis induction
Myricetin-loaded alginate-cellulose hybrid nanocrystals (MAC-NCs)
Selective cytotoxic
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
19 Sep 2024
19 Sep 2024
Historique:
received:
13
05
2024
accepted:
14
08
2024
medline:
20
9
2024
pubmed:
20
9
2024
entrez:
19
9
2024
Statut:
epublish
Résumé
Myricetin, a flavanol present in fruits, tea, and vegetables, has the potential to reduce chronic diseases like gastric cancer by promoting cell death and stopping cell growth. However, its limited bioactivity due to its short lifespan and poor solubility in water has been a challenge. The current research focuses on incorporating myricetin into alginate-cellulose hybrid nanocrystals to enhance its selective proapoptotic effects on human AGS gastric cancer cells. MAC-NCs, myricetin-loaded alginate-cellulose hybrid nanocrystals, were synthesized using a combined co-precipitation/ultrasonic homogenization method and characterized through Dynamic Light Scattering (DLS), Fourier Transform Infrared Spectroscopy (FTIR), Field Emission Scanning Electron Microscope (FESEM), and Zeta-potential analyses. Their cytotoxic activity was tested on cancerous (AGS) and normal (Huvec) cells, revealing selective toxicity. Apoptotic markers, Caspase 8 and Caspase 9, gene expression was measured, and cell death type was confirmed using DAPI staining and flow cytometry on AGS cells. Synthesized MAC-NCs, measuring 40 nm, showed significant selective toxicity on human gastric cells (IC The current study provides critical insights into the therapeutic potential of MAC-NCs for gastric cancer treatment. Based on the notable induction of apoptosis in the AGS cancer cell line, the synthesized MAC-NCs exhibit promising potential as a selective anti-gastric cancer agent. However, further in-vivo studies are necessary to confirm and quantify the nanoparticle's selective toxicity and pharmaceutical properties in future investigations.
Sections du résumé
BACKGROUND
BACKGROUND
Myricetin, a flavanol present in fruits, tea, and vegetables, has the potential to reduce chronic diseases like gastric cancer by promoting cell death and stopping cell growth. However, its limited bioactivity due to its short lifespan and poor solubility in water has been a challenge. The current research focuses on incorporating myricetin into alginate-cellulose hybrid nanocrystals to enhance its selective proapoptotic effects on human AGS gastric cancer cells.
METHODS
METHODS
MAC-NCs, myricetin-loaded alginate-cellulose hybrid nanocrystals, were synthesized using a combined co-precipitation/ultrasonic homogenization method and characterized through Dynamic Light Scattering (DLS), Fourier Transform Infrared Spectroscopy (FTIR), Field Emission Scanning Electron Microscope (FESEM), and Zeta-potential analyses. Their cytotoxic activity was tested on cancerous (AGS) and normal (Huvec) cells, revealing selective toxicity. Apoptotic markers, Caspase 8 and Caspase 9, gene expression was measured, and cell death type was confirmed using DAPI staining and flow cytometry on AGS cells.
RESULTS
RESULTS
Synthesized MAC-NCs, measuring 40 nm, showed significant selective toxicity on human gastric cells (IC
CONCLUSION
CONCLUSIONS
The current study provides critical insights into the therapeutic potential of MAC-NCs for gastric cancer treatment. Based on the notable induction of apoptosis in the AGS cancer cell line, the synthesized MAC-NCs exhibit promising potential as a selective anti-gastric cancer agent. However, further in-vivo studies are necessary to confirm and quantify the nanoparticle's selective toxicity and pharmaceutical properties in future investigations.
Identifiants
pubmed: 39299971
doi: 10.1007/s11033-024-09864-0
pii: 10.1007/s11033-024-09864-0
doi:
Substances chimiques
Alginates
0
Cellulose
9004-34-6
myricetin
76XC01FTOJ
Flavonoids
0
Caspase 9
EC 3.4.22.-
Caspase 8
EC 3.4.22.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
998Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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