Duck hepatitis A virus utilizes PCBP2 to facilitate viral translation and replication.
3Dpol
DHAV-1
IRES
PCBP2
replication
translation
Journal
Veterinary research
ISSN: 1297-9716
Titre abrégé: Vet Res
Pays: England
ID NLM: 9309551
Informations de publication
Date de publication:
19 Sep 2024
19 Sep 2024
Historique:
received:
16
04
2024
accepted:
07
08
2024
medline:
20
9
2024
pubmed:
20
9
2024
entrez:
19
9
2024
Statut:
epublish
Résumé
Duck hepatitis A virus type 1 (DHAV-1) is an important member of the Picornaviridae family that causes highly fatal hepatitis in ducklings. Since picornaviruses have small genomes with limited coding capacity, they must utilize host proteins for viral cap-independent translation and RNA replication. Here, we report the role of duck poly(rC)-binding protein 2 (PCBP2) in regulating the replication and translation of DHAV-1. During DHAV-1 infection, PCBP2 expression was upregulated. A biotinylated RNA pull-down assay revealed that PCBP2 positively regulates DHAV-1 translation through specific interactions with structural domains II and III of the DHAV-1 internal ribosome entry site (IRES). Further studies revealed that PCBP2 promotes DHAV-1 replication via an interaction of its KH1 domain (aa 1-92) with DHAV-1 3D
Identifiants
pubmed: 39300570
doi: 10.1186/s13567-024-01369-9
pii: 10.1186/s13567-024-01369-9
doi:
Substances chimiques
RNA-Binding Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110Subventions
Organisme : China Agriculture Research System
ID : CARS-42-17
Organisme : Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System
ID : SCCXTD-2020-18
Informations de copyright
© 2024. The Author(s).
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