The effect of insect excretions/secretions and decomposition fluid on DNA quantity and quality in human bloodstains.
DNA degradation
blood
decomposition fluid
human DNA
insect excretions/secretions
maggots
Journal
Journal of forensic sciences
ISSN: 1556-4029
Titre abrégé: J Forensic Sci
Pays: United States
ID NLM: 0375370
Informations de publication
Date de publication:
20 Sep 2024
20 Sep 2024
Historique:
revised:
22
07
2024
received:
22
04
2024
accepted:
26
07
2024
medline:
21
9
2024
pubmed:
21
9
2024
entrez:
21
9
2024
Statut:
aheadofprint
Résumé
The larval excretions/secretions (ES) of blowflies contain proteolytic enzymes and bacteria that assist with tissue breakdown. Decomposition fluid (DF) contains organic and inorganic waste products from cell death. This study investigated if human DNA recovery from blood was impacted by exposure to ES and DF over time. Lucilia sericata ES were collected daily from 50 larvae, and all available DF was collected from two fetal piglets left to decompose for 2 weeks. Daily for 3-5 days, 28 μL-30 μL of ES, DF, or a 1:1 mixture of the fluids was added to 30 μL of blood on cotton. Three bloodstains per treatment were sampled every 12 h up to 3 days and at 1 and 2 weeks after initial addition of fluid. No PCR inhibition was detected, but DNA degradation increased over time, primarily in samples exposed to ES and ES/DF mixtures. The amount of DNA recovered decreased over time, but generally more DNA was recovered from DF samples than other samples. Full profiles, or partial profiles suitable for routine database searching (14-39 alleles), were generated from all DF and ES samples and at least one mixture sample at all timepoints. Partial profiles of between 1 and 13 alleles were obtained from all other mixture samples, except one mixture sample which generated no profile. These findings indicate bloodstain evidence recovered from maggot-infested and/or decomposing bodies may generate forensically useful DNA evidence and should be analyzed as quickly as possible after collection or stored appropriately to prevent further degradation.
Identifiants
pubmed: 39305071
doi: 10.1111/1556-4029.15597
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2024 The Author(s). Journal of Forensic Sciences published by Wiley Periodicals LLC on behalf of American Academy of Forensic Sciences.
Références
Zhou C, Byard RW. Factors and processes causing accelerated decomposition in human cadavers–an overview. J Forensic Leg Med. 2011;18:6–9. https://doi.org/10.1016/j.jflm.2010.10.003
Collins S, Stuart B, Ueland M. Monitoring human decomposition products collected in clothing: an infrared spectroscopy study. Aust J Forensic Sci. 2020;52(4):428–438. https://doi.org/10.1080/00450618.2019.1593504
Sirker M, Schneider PM, Gomes I. A 17‐month time course study of human RNA and DNA degradation in body fluids under dry and humid environmental conditions. Int J Leg Med. 2016;130:1431–1438. https://doi.org/10.1007/s00414‐016‐1373‐9
Alaeddini R, Walsh SJA, Abbas A. Forensic implications of genetic analyses from degraded DNA—A review. Forensic Sci Int Genet. 2010;4:148–157. https://doi.org/10.1016/j.fsigen.2009.09.007
Emmons AL, DeBruyn JM, Mundorff AZ, Cobaugh KL, Cabana GS. The persistence of human DNA in soil following surface decomposition. Sci Justice. 2017;57(5):341–348. https://doi.org/10.1016/j.scijus.2017.05.002
Kanvah S, Joseph J, Schuster GB, Barnett RN, Cleveland CL, Landman U. Oxidation of DNA: damage to nucleobases. Acc Chem Res. 2010;43:280–287. https://doi.org/10.1021/ar900175a
Amendt J, Richards CS, Campobasso CP, Zehner R, Hall MJ. Forensic entomology: applications and limitations. Forensic Sci Med Pathol. 2011;7:379–392. https://doi.org/10.1007/s12024‐010‐9209‐2
Bexfield A, Nigam Y, Thomas S, Ratcliffe NA. Detection and partial characterisation of two antibacterial factors from the excretions/secretions of the medicinal maggot Lucilia sericata and their activity against methicillin‐resistant Staphylococcus aureus (MRSA). Microbes Infect. 2004;6:1297–1304. https://doi.org/10.1016/j.micinf.2004.08.011
Pinilla YT, Moreno‐Perez DA, Patarroyo MA, Bello FJ. Proteolytic activity regarding Sarconesiopsis magellanica (Diptera: Calliphoridae) larval excretions and secretions. Acta Trop. 2013;128:686–691. https://doi.org/10.1016/j.actatropica.2013.09.020
Waldner BJ, Kraml J, Kahler U, Spinn A, Schauperl M, Podewitz M. Electrostatic recognition in substrate binding to serine proteases. J Mol Recognit. 2018;31:e2727. https://doi.org/10.1002/jmr.2727
Stolerman LM, Getz M, Smith SGL, Rangamani P, Holst M. Stability analysis of a bulk‐surface reaction model for membrane protein clustering. Bull Math Biol. 2020;82(2):30. https://doi.org/10.1007/s11538‐020‐00703‐4
Solanki P, Putatunda C, Kumar A, Bhatia R, Walia A. Microbial proteases: ubiquitous enzymes with innumerable uses. 3 Biotech. 2021;11(10):428. https://doi.org/10.1007/s13205‐021‐02928‐z
Dissing J, Sondervang A, Lund S. Exploring the limits for survival of DNA in blood stains. J Forensic Leg Med. 2010;17:392–396. https://doi.org/10.1016/j.jflm.2010.08.001
Durdle A, Mitchell RJ, van Oorschot RAH. The change in human DNA content over time in the artefacts of the blowfly Lucilia cuprina (Meigen) (Diptera: Calliphoridae). Forensic Sci Int Genet Suppl Ser. 2011;3(1):e289–e290. https://doi.org/10.1016/j.fsigss.2011.09.007
Durdle A, van Oorschot RAH, Mitchell RJ. The human DNA content of artifacts deposited by the blowfly Lucilia cuprina fed human blood, semen and saliva. Forensic Sci Int. 2013;233:212–219. https://doi.org/10.1016/j.forsciint.2013.09.015
Bowdle B, Veth J. Review of PowerPlex® 21 & STRmix™ v1.05 Validations. Queensland: The Commission of Inquiry into Forensic DNA Testing in Queensland; 2022. https://www.dnainquiry.qld.gov.au/public‐hearings/assets/exhibits/module‐6/Exh%20219.pdf Accessed 26 Jul 2024.
Terra WR, Ferreira C. Insect digestive enzymes: properties, compartmentalization and function. Comp Biochem Physiol. 1994;109B(1):1–62. https://doi.org/10.1016/0305‐0491(94)90141‐4
Mumcuoglu KY, Miller J, Mumcuoglu M, Friger M, Tarshis M. Destruction of bacteria in the digestive tract of the maggot of Lucilia sericata (Diptera: Calliphoridae). J Med Entol. 2001;38(2):161–166. https://doi.org/10.1603/0022‐2585‐38.2.161
Hofer IMJ, Hart AJ, Martin‐Vega D, Hall MJR. Age estimation during the blow fly intra‐puparial period. Forensic Sci Int. 2017;270:129–138. https://doi.org/10.1007/s00414‐017‐1598‐2
Hyde ER, Haarmann DP, Lynne AM, Bucheli SR, Petrosino JF. The living dead: bacterial community structure of a cadaver at the onset and end of the bloat stage of decomposition. PLoS One. 2013;8(10):e77733. https://doi.org/10.1371/journal.pone.0077733
Kouadio KJ, Kouadio KKA, Koffi AF, Kouassi KF, Aboua LRN, Beugre J‐B. Diet and rate of decomposition of the corpse in a human surrogate. Int J Leg Med. 2024;133:43–53. https://doi.org/10.1007/s00414‐022‐02877‐z
Connor M, Baigent C, Hansen ES. Testing the use of pigs as human proxies in decomposition studies. J Forensic Sci. 2018;63(5):1350–1355. https://doi.org/10.1111/1556‐4029.13727
Banin E, Brady KM, Greenberg EP. Chelator‐induced dispersal and killing of Pseudomonas aeruginosa cells in a biofilm. Appl Environ Microbiol. 2006;72(3):2064–2069. https://doi.org/10.1128/AEM.72.3.2064‐2069.2006
Chang Y, Gu W, McLandsborough L. Low concentration of ethylenediaminetetraacetic acid (EDTA) affects biofilm formation of listeria monocytogenes by inhibiting its initial adherence. Food Microbiol. 2012;29(1):10–17. https://doi.org/10.1016/j.fm.2011.07.009
Hughes D, van Oorschot RAH, Szkuta B, Conlan XA. The effect of anti‐coagulant EDTA on the deposition and adhesion of whole blood deposits on non‐porous substrates. J Forensic Sci. 2024;69(3):1061–1068. https://doi.org/10.1111/1556‐4029.15483