Anti-tumor potential of high salt in breast Cancer cell lines.
Humans
Breast Neoplasms
/ drug therapy
Female
Apoptosis
/ drug effects
Cell Proliferation
/ drug effects
Cell Movement
/ drug effects
MCF-7 Cells
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
/ drug effects
Cell Adhesion
/ drug effects
Cell Cycle
/ drug effects
Tumor Suppressor Protein p53
/ metabolism
Antineoplastic Agents
/ pharmacology
Sodium Chloride
/ pharmacology
Cell Cycle Checkpoints
/ drug effects
Adhesion
Apoptosis
Breast cancer
Cell cycle
Cell proliferation
High salt
MCF-7
MDA MB-231
Migration
RNA-sequencing
Wound healing
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
21 Sep 2024
21 Sep 2024
Historique:
received:
29
06
2024
accepted:
09
09
2024
medline:
21
9
2024
pubmed:
21
9
2024
entrez:
21
9
2024
Statut:
epublish
Résumé
Recent Here, the impact of high salt on apoptosis, proliferation, cell cycle, adhesion, and migration of MDA-MB-231 and MCF-7 cells was studied using MTT, scratch, and clonogenic assays, as well as RT-PCR and flow cytometry. Gene expression was analyzed using Real-Time PCR and western blotting. The effect of high salt on global transcriptomics changes in MDA MB-231 cells was studied using RNA-sequencing analysis. Flow cytometry with Annexin V and CFSE revealed that high salt-induced dose-dependent apoptosis and inhibited proliferation. High salt-induced cell cycle arrest at the G1/S phase of the cell cycle. p-MDM2 is known to suppress p53, which plays a crucial role in regulating apoptosis and cell cycle arrest under cellular stress conditions. High salt treatment led to decreased p-MDM2 and increased p53 expression, suggesting that high salt induces apoptosis through p53 stabilization. decreased p-MDM2 and increased p53 expression. High salt also reduced migration and adhesion of cells in a dose-dependent manner suggesting its inhibitory effect on metastatic properties as evident from wound healing assay. RNA sequencing analysis revealed overexpression of tumor suppressor genes and genes associated with anti-tumor activity (PCDHGA11, EIF3CL, RAVER1, TNFSF15, RANBP3L) and under-expression of genes involved in cancer-promoting activity (MT1X, CLDN14, CSF-2). Our results unequivocally demonstrate the anti-tumor efficacy of high salt against breast cancer cells, suggesting its potential as a therapeutic strategy in cancer treatment.
Sections du résumé
BACKGROUND
BACKGROUND
Recent
METHODS
METHODS
Here, the impact of high salt on apoptosis, proliferation, cell cycle, adhesion, and migration of MDA-MB-231 and MCF-7 cells was studied using MTT, scratch, and clonogenic assays, as well as RT-PCR and flow cytometry. Gene expression was analyzed using Real-Time PCR and western blotting. The effect of high salt on global transcriptomics changes in MDA MB-231 cells was studied using RNA-sequencing analysis.
RESULTS
RESULTS
Flow cytometry with Annexin V and CFSE revealed that high salt-induced dose-dependent apoptosis and inhibited proliferation. High salt-induced cell cycle arrest at the G1/S phase of the cell cycle. p-MDM2 is known to suppress p53, which plays a crucial role in regulating apoptosis and cell cycle arrest under cellular stress conditions. High salt treatment led to decreased p-MDM2 and increased p53 expression, suggesting that high salt induces apoptosis through p53 stabilization. decreased p-MDM2 and increased p53 expression. High salt also reduced migration and adhesion of cells in a dose-dependent manner suggesting its inhibitory effect on metastatic properties as evident from wound healing assay. RNA sequencing analysis revealed overexpression of tumor suppressor genes and genes associated with anti-tumor activity (PCDHGA11, EIF3CL, RAVER1, TNFSF15, RANBP3L) and under-expression of genes involved in cancer-promoting activity (MT1X, CLDN14, CSF-2).
CONCLUSION
CONCLUSIONS
Our results unequivocally demonstrate the anti-tumor efficacy of high salt against breast cancer cells, suggesting its potential as a therapeutic strategy in cancer treatment.
Identifiants
pubmed: 39305332
doi: 10.1007/s11033-024-09925-4
pii: 10.1007/s11033-024-09925-4
doi:
Substances chimiques
Tumor Suppressor Protein p53
0
Antineoplastic Agents
0
Sodium Chloride
451W47IQ8X
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1002Subventions
Organisme : Indian Council of Medical Research
ID : Sanction No.3/2/2/29/2022-NCD-III
Organisme : Science and Engineering Research Board
ID : CRG/2021/008212
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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