Bacteriocin Antagonistic Potentials of Lactococcus cremoris and Lactococcus lactis Isolates from Different Habitats.

Antagonism Comparative genomics Lactococci Lactococcin B Niche adaptation Nisin

Journal

Probiotics and antimicrobial proteins

ISSN: 1867-1314

Titre abrégé: Probiotics Antimicrob Proteins

Pays: United States

ID NLM: 101484100

Informations de publication

Date de publication:
21 Sep 2024

Historique:

accepted: 04 09 2024

medline: 21 9 2024

pubmed: 21 9 2024

entrez: 21 9 2024

Statut: aheadofprint

Résumé

It has not been extensively examined if the ecological role of lactic acid bacterial bacteriocins may affect their distribution in different habitats and thereby impact screenings for novel variants. Further, the functionality of such variants requires additional investigation. This study investigated the distribution of bacteriocin biosynthetic gene clusters (BGCs) and bacteriocinogenic activity of Lactococcus cremoris and Lactococcus lactis from a range of different environments. Whole genome sequencing and phylogenetic analysis of fifty L. cremoris and L. lactis strains showed distinct species clustering without significant genome size differences between species or sources. Genomic screening with AntiSMASH and BAGEL4 identified several BGCs, with variation based on species but not on habitat. Deferred inhibition assays revealed pronounced activity only in strains possessing nisin or lactococcin B BGCs and not in strains with other BGCs. Several hitherto undescribed types of lactococcin BGCs appeared to be incomplete regarding genes encoding secretion and immunity. In conclusion, this study indicates that habitats did not appear to affect distribution of BGCs. Further, the antagonistic functionality of several BGCs was unclear indicating that improving applications of lactococcal bacteriocins may depend as much or more on research on increasing efficacy of well-known bacteriocins than searching for novel variants.

Identifiants

DOI: 10.1007/s12602-024-10361-w PMID: 39305435

pubmed: 39305435

doi: 10.1007/s12602-024-10361-w

pii: 10.1007/s12602-024-10361-w

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Subventions

Organisme : China Scholarship Council

ID : CSC No. 202006350030

Organisme : Norges Forskningsråd

ID : FRIPRO 314490

Informations de copyright

Références

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