Blastocystis species growth inhibition by plant extracts in vitro assays.
Blastocystis species
Cytotoxicity
In vitro
Plant extracts
Subtypes
Journal
Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191
Informations de publication
Date de publication:
20 Sep 2024
20 Sep 2024
Historique:
received:
02
05
2024
revised:
05
08
2024
accepted:
19
09
2024
medline:
23
9
2024
pubmed:
23
9
2024
entrez:
22
9
2024
Statut:
aheadofprint
Résumé
The protist Blastocystis sp. inhabits the gastrointestinal tracts of humans and animals. In recent decades, alternative natural products derived from plants have demonstrated potential as effective treatments for Blastocystis infection. The anti-Blastocystis activity of three herbal ethanolic extracts- Odontites linkii subsp. cyprius, Ptilostemon chamaepeuce subsp. cyprius and Quercus alnifolia-were investigated in this study. Three distinct isolates of Blastocystis sp. maintained in vitro were molecularly subtyped. Cytotoxicity analysis was performed on individual Blastocystis sp. isolates using 250, 500, 1,000, and 2,000 μg/mL herbal ethanolic extracts for 24 and 48 hours. Quantitative, morphological, and size alterations of Blastocystis cells assessed the cytotoxicity of herbal anti-Blastocystis effect. Following subtyping analysis, one strain of Blastocystis had ST3 and ST1 mixed subtypes, and two strains had ST1 subtypes. Starting after 24 hours of incubation, P. cham. subsp. cyprius (1,000 μg/mL) exhibited the most pronounced and consistent anti-Blastocystis cytotoxicity against all three strains, comparable to metronidazole. The Ptilostemon chamaepeuce subsp. cyprius anti-Blastocystis cytotoxicity was evident in parasite quantitative distress, morphological alterations, and significant reductions in cell size. Odontites linkii subsp. cyprius cytotoxicity varied among the three Blastocystis strains. The three Blastocystis strains were resistant to Quercus alnifolia. P. cham. subsp. cyprius was a potent and promising new herbal extract against Blastocystis sp. in vitro assays.
Sections du résumé
BACKGROUND
BACKGROUND
The protist Blastocystis sp. inhabits the gastrointestinal tracts of humans and animals. In recent decades, alternative natural products derived from plants have demonstrated potential as effective treatments for Blastocystis infection. The anti-Blastocystis activity of three herbal ethanolic extracts- Odontites linkii subsp. cyprius, Ptilostemon chamaepeuce subsp. cyprius and Quercus alnifolia-were investigated in this study.
METHODS
METHODS
Three distinct isolates of Blastocystis sp. maintained in vitro were molecularly subtyped. Cytotoxicity analysis was performed on individual Blastocystis sp. isolates using 250, 500, 1,000, and 2,000 μg/mL herbal ethanolic extracts for 24 and 48 hours. Quantitative, morphological, and size alterations of Blastocystis cells assessed the cytotoxicity of herbal anti-Blastocystis effect.
RESULTS
RESULTS
Following subtyping analysis, one strain of Blastocystis had ST3 and ST1 mixed subtypes, and two strains had ST1 subtypes. Starting after 24 hours of incubation, P. cham. subsp. cyprius (1,000 μg/mL) exhibited the most pronounced and consistent anti-Blastocystis cytotoxicity against all three strains, comparable to metronidazole. The Ptilostemon chamaepeuce subsp. cyprius anti-Blastocystis cytotoxicity was evident in parasite quantitative distress, morphological alterations, and significant reductions in cell size. Odontites linkii subsp. cyprius cytotoxicity varied among the three Blastocystis strains. The three Blastocystis strains were resistant to Quercus alnifolia.
CONCLUSION
CONCLUSIONS
P. cham. subsp. cyprius was a potent and promising new herbal extract against Blastocystis sp. in vitro assays.
Identifiants
pubmed: 39307197
pii: S0882-4010(24)00437-6
doi: 10.1016/j.micpath.2024.106970
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
106970Informations de copyright
Copyright © 2024. Published by Elsevier Ltd.
Déclaration de conflit d'intérêts
Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. ☐ The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: