Adaptor protein Src-homology 2 domain containing E (SH2E) deficiency induces heart defect in zebrafish.

CRISPR/Cas9 Src-homology 2 domain containing E cardiac development pericardial edema zebrafish

Journal

Acta pharmacologica Sinica
ISSN: 1745-7254
Titre abrégé: Acta Pharmacol Sin
Pays: United States
ID NLM: 100956087

Informations de publication

Date de publication:
23 Sep 2024
Historique:
received: 03 05 2024
accepted: 05 09 2024
revised: 04 09 2024
medline: 24 9 2024
pubmed: 24 9 2024
entrez: 23 9 2024
Statut: aheadofprint

Résumé

Adaptor proteins play crucial roles in signal transduction across diverse signaling pathways. Src-homology 2 domain-containing E (SH2E) is the adaptor protein highly expressed in vascular endothelial cells and myocardium during zebrafish embryogenesis. In this study we investigated the function and mechanisms of SH2E in cardiogenesis. We first analyzed the spatiotemporal expression of SH2E and then constructed zebrafish lines with SH2E deficiency using the CRISPR-Cas9 system. We showed that homozygous mutants developed progressive pericardial edema (PCE), dilated atrium, abnormal atrioventricular looping and thickened atrioventricular wall from 3 days post fertilization (dpf) until death; inducible overexpression of SH2E was able to partially rescue the PCE phenotype. Using transcriptome sequencing analysis, we demonstrated that the MAPK/ERK and NF-κB signaling pathways might be involved in SH2E-deficiency-caused PCE. This study underscores the pivotal role of SH2E in cardiogenesis, and might help to identify innovative diagnostic techniques and therapeutic strategies for congenital heart disease.

Identifiants

DOI: 10.1038/s41401-024-01392-8 PMID: 39313516
pubmed: 39313516
doi: 10.1038/s41401-024-01392-8
pii: 10.1038/s41401-024-01392-8
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2024. The Author(s), under exclusive licence to Shanghai Institute of Materia Medica, Chinese Academy of Sciences and Chinese Pharmacological Society.

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Auteurs

Yu-Lai Liang (YL)

Laboratory of Molecular Immunology, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200438, China.
CAS Key Laboratory of Tissue Microenvironment and Tumor, Innovation Center for Intervention of Chronic Disease and Promotion of Health, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.

Yang-Xi Hu (YX)

Department of Cardiology, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China.
Department of Pharmacy, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China.

Fang-Fang Li (FF)

Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China. lifang091001@163.com.

Hong-Min You (HM)

Department of Cardiovascular Medicine, Changhai Hospital, Naval Medical University, Shanghai, 200433, China.

Jian Chen (J)

CAS Key Laboratory of Tissue Microenvironment and Tumor, Innovation Center for Intervention of Chronic Disease and Promotion of Health, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.

Chun Liang (C)

Department of Cardiology, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China.

Zhi-Fu Guo (ZF)

Department of Cardiovascular Medicine, Changhai Hospital, Naval Medical University, Shanghai, 200433, China.

Qing Jing (Q)

CAS Key Laboratory of Tissue Microenvironment and Tumor, Innovation Center for Intervention of Chronic Disease and Promotion of Health, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China. qjing@sibs.ac.cn.

Classifications MeSH