Cold atmospheric plasma activated media selectively affects human head and neck cancer cell lines.
apoptosis
cell proliferation
cold atmospheric plasma
head and neck cancer
ionic chromatography
plasma activated media
Journal
Oral diseases
ISSN: 1601-0825
Titre abrégé: Oral Dis
Pays: Denmark
ID NLM: 9508565
Informations de publication
Date de publication:
24 Sep 2024
24 Sep 2024
Historique:
revised:
24
07
2024
received:
14
12
2023
accepted:
11
08
2024
medline:
24
9
2024
pubmed:
24
9
2024
entrez:
24
9
2024
Statut:
aheadofprint
Résumé
Cold atmospheric plasma (CAP) is a novel approach for cancer treatment. It can be used to treat liquids-plasma-activated media (PAM)-which are then transferred to the target as an exogenous source of reactive oxygen and nitrogen species (RONS). The present study aimed at chemically characterizing different PAM and assessing their in vitro selectivity against head and neck cancer cells (HNC). PAM were obtained by exposing 2 and 5 mL of cell culture medium to CAP for 5, 10 and 20 min at a 6 mm working distance. Anions kinetics was evaluated by ion chromatography. Cell proliferation inhibition, apoptosis occurrence, and cell cycle modifications were assessed by MTS and flow cytometry, on human epidermal keratinocyte (HaCaT) and HNC cell lines HSC3, HSC4 and A253. The 2 mL conditions showed a significant reduction in cell proliferation whereas for the 5 mL the effect was milder, but the time-dependence was more evident. HaCaT were unaffected by the 5 mL PAM, indicating a selectivity for cancer cells. The media chemical composition modified by CAP exposure influenced cell proliferation by modulating cell cycle and inducing apoptosis in cancer cells, without affecting normal cells.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Union-NextGenerationEU-MUR, DM 737/2021
Organisme : Fondazione AIRC
ID : IG
Organisme : Fondazione AIRC
ID : 2021
Organisme : Fondazione AIRC
ID : id25696
Organisme : National Research Foundation (NRF) of Korea, funded by the Korean government
ID : 2021R1A6A1A03038785
Informations de copyright
© 2024 The Author(s). Oral Diseases published by John Wiley & Sons Ltd.
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