Low penetrance of frequent ATP7B mutations explains the low prevalence of Wilson disease. Lessons from real-life registries.

Genetic screening Genotype-phenotype correlation Misdiagnosis Rare liver disease

Journal

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver
ISSN: 1878-3562
Titre abrégé: Dig Liver Dis
Pays: Netherlands
ID NLM: 100958385

Informations de publication

Date de publication:
24 Sep 2024
Historique:
received: 09 05 2024
revised: 03 08 2024
accepted: 03 09 2024
medline: 26 9 2024
pubmed: 26 9 2024
entrez: 25 9 2024
Statut: aheadofprint

Résumé

Wilson disease (WD) is a copper metabolism disorder caused by mutations in ATP7B gene, with significant clinical variability. Several studies have analyzed the prevalence and penetrance of mutations. We evaluated both characteristics for our more frequent mutations. Evaluation of 260 patients from the National Registry: clinical, analytical and genetic data. Estimation of homozygotes and total cases according to Hardy-Weinberg equilibrium and comparison with Registry records. The estimated number of homozygotes were higher than registered: p.Met645Arg (1949/6), p.His1069Gln (20/8), p.Leu708Pro (63/24) and p.Gly869Arg (147/0). p.Met645Arg homozygotes presented less cirrhosis at diagnosis, extrahepatic disease and Kayser-Fleischer ring (KFR) and more presymptomatic cases and diagnosis after 40 years of age than p.Leu708Pro and p.His1069Gln homozygotes. p.Met645Arg homozygotes presented more late diagnosis than p.Met645Arg compound heterozygotes. Compound heterozygotes carrying p.Met645Arg or p.Gly869Arg showed less cirrhosis at diagnosis, KFR and neurological symptoms and more hepatic and presymptomatic cases, despite clearly low ceruloplasmin levels. The estimated prevalence was 1:3.785, predicting more than 10.500 patients. The widespread mutations p.Met645Arg and p.Gly869Arg show low penetrance. WD might be underdiagnosed in Spain due to less severe phenotype of the most frequent mutations, a crucial fact to avoid misdiagnosis and to offer early therapy.

Sections du résumé

BACKGROUND & AIMS OBJECTIVE
Wilson disease (WD) is a copper metabolism disorder caused by mutations in ATP7B gene, with significant clinical variability. Several studies have analyzed the prevalence and penetrance of mutations. We evaluated both characteristics for our more frequent mutations.
METHODS METHODS
Evaluation of 260 patients from the National Registry: clinical, analytical and genetic data. Estimation of homozygotes and total cases according to Hardy-Weinberg equilibrium and comparison with Registry records.
RESULTS RESULTS
The estimated number of homozygotes were higher than registered: p.Met645Arg (1949/6), p.His1069Gln (20/8), p.Leu708Pro (63/24) and p.Gly869Arg (147/0). p.Met645Arg homozygotes presented less cirrhosis at diagnosis, extrahepatic disease and Kayser-Fleischer ring (KFR) and more presymptomatic cases and diagnosis after 40 years of age than p.Leu708Pro and p.His1069Gln homozygotes. p.Met645Arg homozygotes presented more late diagnosis than p.Met645Arg compound heterozygotes. Compound heterozygotes carrying p.Met645Arg or p.Gly869Arg showed less cirrhosis at diagnosis, KFR and neurological symptoms and more hepatic and presymptomatic cases, despite clearly low ceruloplasmin levels. The estimated prevalence was 1:3.785, predicting more than 10.500 patients.
CONCLUSIONS CONCLUSIONS
The widespread mutations p.Met645Arg and p.Gly869Arg show low penetrance. WD might be underdiagnosed in Spain due to less severe phenotype of the most frequent mutations, a crucial fact to avoid misdiagnosis and to offer early therapy.

Identifiants

pubmed: 39322449
pii: S1590-8658(24)01000-4
doi: 10.1016/j.dld.2024.09.002
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2024 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest Victor Vargas: Speaker fees, Alexion Pharma, Consultant (advisory board), Ipsen. Anna Miralpeix: speaker fees from Orphalan. Zoe Mariño: speaker fees from Orphalan and Gilead; advisory fees from Orphalan, Alexion and Deep Genomics; grants from Gilead. The others authors reported no conflict of interest.

Auteurs

Pablo Alonso-Castellano (P)

Universidad Las Palmas Gran Canaria. Servicio Digestivo, Complejo Hospitalario Universitario Insular Materno Infantil (CHUIMI), Las Palmas de Gran Canaria, Spain. Electronic address: pabloalonsoc1997@gmail.com.

Antonio Tugores (A)

Unidad Apoyo Investigación, CHUIMI, Las Palmas de Gran Canaria, Spain. Electronic address: atugores@yahoo.com.

Zoe Mariño (Z)

LiverUnit, Hospital Clínic, Universitat de Barcelona, Barcelona, Spain. Electronic address: zmarino@clinic.cat.

Antonio Olveira (A)

Servicio Digestivo, Hospital Universitario La Paz, Madrid, Spain. Electronic address: aolveiram@gmail.com.

Marina Berenguer (M)

Hospital Universitari i Politècnic La Fe, IISLaFe, Universidad de Valencia y Ciberehd, Valencia, Spain. Electronic address: marina.berenguer@uv.es.

M Pilar Huarte (MP)

Complejo Hospitalario de Navarra, Pamplona, Spain. Electronic address: phuartem@navarra.es.

Jose R Fernández-Ramos (JR)

Hospital Universitario de Cruces, Baracaldo, Spain. Electronic address: joseramon.fernandezramos@osakidetza.eus.

María Lázaro-Ríos (M)

Hospital Universitario Miguel Servet, Zaragoza, Spain. Electronic address: marialazarorios@hotmail.com.

María L González-Diéguez (ML)

Hospital Universitario Central de Asturias, Oviedo, Spain. Electronic address: luisagondi@hotmail.com.

José M Moreno-Planas (JM)

Servicio de Aparato Digestivo, Complejo Hospitalario Universitario de Albacete, Facultad de Medicina Universidad de Castilla La Mancha, Spain. Electronic address: josemariamoren@yahoo.es.

Manuel Hernández-Guerra (M)

Hospital Universitario de Canarias, Santa Cruz Tenerife, Spain. Electronic address: mhernand@ull.edu.es.

Paula Fernández-Álvarez (P)

Hospital Universitario Virgen Macarena, Sevilla, Spain. Electronic address: paulafer7@gmail.com.

Manuel Delgado-Blanco (M)

Hospital Universitario A Coruña, A Coruña, Spain. Electronic address: manuel.delgado.blanco@sergas.es.

José M Pinazo-Bandera (JM)

Unidad de Hepatología, Unidad de Gestión Clínica de Aparato Digestivo, Hospital Universitario Virgen de la Victoria, Instituto de Investigación Biomédica de Málaga-Plataforma Bionand, Málaga, Spain. Electronic address: Josepinazo@hotmail.es.

Marta Romero (M)

Hospital Universitario de Toledo, Toledo, Spain. Electronic address: m.romero.gutierrez@gmail.com.

Javier Ampuero (J)

Hospital Universitario Virgen del Rocío, Sevilla, Spain. Electronic address: jampuero-ibis@us.es.

Helena Masnou-Ridaura (H)

Hospital UniversitariGermansTrias i Pujol, Badalona, Spain. Electronic address: hmasnou.germanstrias@gencat.cat.

Alba Cachero (A)

Hospital Universitario de Bellvitge, Hospitalet de Llobregat, Spain. Electronic address: alba.cachero@bellvitgehospital.cat.

Víctor Vargas (V)

LiverUnit, Hospital Vall d'Hebron, Universitat Autónoma Barcelona, CIBERehd, Barcelona, Spain. Electronic address: victor.vargas@uab.cat.

Judith Gómez-Camarero (J)

Hospital Universitario de Burgos, Burgos, Spain. Electronic address: jgomcam@hotmail.com.

María J Morillas-Ariño (MJ)

Hospital Universitario Virgen de la Luz, Cuenca, Spain. Electronic address: Julmorar@hotmail.com.

Esther Molina-Pérez (E)

Hospital Clínico de Santiago, Santiago de Compostela, Spain. Electronic address: esther.molina.perez@sergas.es.

Anna Miralpeix (A)

LiverUnit, Hospital Clínic, Universitat de Barcelona, Barcelona, Spain. Electronic address: amiralpeix@recerca.clinic.cat.

Luis García-Villarreal (L)

Grupo de Investigación Patología Médica, Instituto de Investigaciones Biomédicas y Sanitarias. Universidad de Las Palmas de Gran Canaria. Servicio Digestivo, Complejo Hospitalario Universitario Insular Materno Infantil (CHUIMI), Las Palmas de Gran Canaria, Spain. Electronic address: lgarciavillarreal@gmail.com.

Classifications MeSH