Single-cell sequencing suggests a conserved function of Hedgehog-signalling in spider eye development.
Arthropod evolution
Arthropod head development
Eye development
Visual system development
Journal
EvoDevo
ISSN: 2041-9139
Titre abrégé: Evodevo
Pays: England
ID NLM: 101525836
Informations de publication
Date de publication:
26 Sep 2024
26 Sep 2024
Historique:
received:
02
02
2024
accepted:
01
09
2024
medline:
27
9
2024
pubmed:
27
9
2024
entrez:
26
9
2024
Statut:
epublish
Résumé
Spiders evolved different types of eyes, a pair of primary eyes that are usually forward pointing, and three pairs of secondary eyes that are typically situated more posterior and lateral on the spider's head. The best understanding of arthropod eye development comes from the vinegar fly Drosophila melanogaster, the main arthropod model organism, that also evolved different types of eyes, the larval eyes and the ocelli and compound eyes of the imago. The gene regulatory networks that underlie eye development in this species are well investigated revealing a conserved core network, but also show several differences between the different types of eyes. Recent candidate gene approaches identified a number of conserved genes in arthropod eye development, but also revealed crucial differences including the apparent lack of some key factors in some groups of arthropods, including spiders. Here, we re-analysed our published scRNA sequencing data and found potential key regulators of spider eye development that were previously overlooked. Unlike earlier research on this topic, our new data suggest that Hedgehog (Hh)-signalling is involved in eye development in the spider Parasteatoda tepidariorum. By investigating embryonic gene expression in representatives of all main groups of spiders, we demonstrate that this involvement is conserved in spiders. Additionally, we identified genes that are expressed in the developing eyes of spiders, but that have not been studied in this context before. Our data show that single-cell sequencing represents a powerful method to gain deeper insight into gene regulatory networks that underlie the development of lineage-specific organs such as the derived set of eyes in spiders. Overall, we gained deeper insight into spider eye development, as well as the evolution of arthropod visual system formation.
Sections du résumé
BACKGROUND
BACKGROUND
Spiders evolved different types of eyes, a pair of primary eyes that are usually forward pointing, and three pairs of secondary eyes that are typically situated more posterior and lateral on the spider's head. The best understanding of arthropod eye development comes from the vinegar fly Drosophila melanogaster, the main arthropod model organism, that also evolved different types of eyes, the larval eyes and the ocelli and compound eyes of the imago. The gene regulatory networks that underlie eye development in this species are well investigated revealing a conserved core network, but also show several differences between the different types of eyes. Recent candidate gene approaches identified a number of conserved genes in arthropod eye development, but also revealed crucial differences including the apparent lack of some key factors in some groups of arthropods, including spiders.
RESULTS
RESULTS
Here, we re-analysed our published scRNA sequencing data and found potential key regulators of spider eye development that were previously overlooked. Unlike earlier research on this topic, our new data suggest that Hedgehog (Hh)-signalling is involved in eye development in the spider Parasteatoda tepidariorum. By investigating embryonic gene expression in representatives of all main groups of spiders, we demonstrate that this involvement is conserved in spiders. Additionally, we identified genes that are expressed in the developing eyes of spiders, but that have not been studied in this context before.
CONCLUSION
CONCLUSIONS
Our data show that single-cell sequencing represents a powerful method to gain deeper insight into gene regulatory networks that underlie the development of lineage-specific organs such as the derived set of eyes in spiders. Overall, we gained deeper insight into spider eye development, as well as the evolution of arthropod visual system formation.
Identifiants
pubmed: 39327634
doi: 10.1186/s13227-024-00230-6
pii: 10.1186/s13227-024-00230-6
doi:
Types de publication
Journal Article
Langues
eng
Pagination
11Subventions
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 766053
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 766053
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 766053
Organisme : Vetenskapsrådet
ID : 2022-03522
Organisme : Vetenskapsrådet
ID : 2022-03522
Organisme : Deutsche Forschungsgemeinschaft
ID : PE 2075/4-1
Organisme : Deutsche Forschungsgemeinschaft
ID : PO 1648/6-1
Informations de copyright
© 2024. The Author(s).
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